ArticlePloS one2011
Site-specific integration and expression of an anti-malarial gene in transgenic Anopheles gambiae significantly reduces Plasmodium infections.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 109 citations in OpenAlex.
- Killer bee molecules: antimicrobial peptides as effector molecules to target sporogonic stages of Plasmodium.PLoS pathogens · 2013Trial
- Mosquito-based malaria control through genetic, microbial and immunological innovations.Nature reviews. Microbiology · 2026Review
- An OpIE2-DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector Anopheles gambiae.Genetics · 2026Article
- ImprovedInternational journal of molecular sciences · 2023Article
- Use of Insect Promoters in Genetic Engineering to Control Mosquito-Borne Diseases.Biomolecules · 2022Review
- Gene drive mosquitoes can aid malaria elimination by retardingScience advances · 2022Article
- Population replacement gene drive characteristics for malaria elimination in a range of seasonal transmission settings: a modelling study.Malaria journal · 2022Article
- Testing non-autonomous antimalarial gene drive effectors using self-eliminating drivers in the African mosquito vector Anopheles gambiae.PLoS genetics · 2022Article
- Transgenic Anopheles mosquitoes expressing human PAI-1 impair malaria transmission.Nature communications · 2022Article
- Article
- Multiple blood feeding in mosquitoes shortens the Plasmodium falciparum incubation period and increases malaria transmission potential.PLoS pathogens · 2020Article
- Heterogeneous expression of the ammonium transporter AgAmt in chemosensory appendages of the malaria vector, Anopheles gambiae.Insect biochemistry and molecular biology · 2020Article
- Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila.Insect biochemistry and molecular biology · 2020Article
- Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes.Parasites & vectors · 2018Review
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR.G3 (Bethesda, Md.) · 2018Article
- Modern Vector Control.Cold Spring Harbor perspectives in medicine · 2018Review
- Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?International journal of environmental research and public health · 2017Review
- Cre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti.Scientific reports · 2017Article
- Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe.Applied and environmental microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 3 countries.
Funding
Abstract
Diseases transmitted by mosquitoes have a devastating impact on global health and this is worsening due to difficulties with existing control measures and climate change. Genetically modified mosquitoes that are refractory to disease transmission are seen as having great potential in the delivery of novel control strategies. Historically the genetic modification of insects has relied upon transposable elements which have many limitations despite their successful use. To circumvent these limitations the Streptomyces phage phiC31 integrase system has been successfully adapted for site-specific transgene integration in insects. Here, we present the first site-specific transformation of Anopheles gambiae, the principal vector of human malaria. Mosquitoes were initially engineered to incorporate the phiC31 targeting site at a defined genomic location. A second phase of genetic modification then achieved site-specific integration of Vida3, a synthetic anti-malarial gene. Expression of Vida3, specifically in the midgut of bloodfed females, offered consistent and significant protection against Plasmodium yoelii nigeriensis, reducing average parasite intensity by 85%. Similar protection was observed against Plasmodium falciparum in some experiments, although protection was inconsistent. In the fight against malaria, it is imperative to establish a broad repertoire of both anti-malarial effector genes and tissue-specific promoters for their expression, enabling those offering maximum effect with minimum fitness cost to be identified. In the future, this technology will allow effective comparisons and informed choices to be made, potentially leading to complete transmission blockade.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.