ArticlePloS one2011
Isolation of a rickettsial pathogen from a non-hematophagous arthropod.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 60 citations in OpenAlex.
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- Cat Flea Coinfection withVector borne and zoonotic diseases (Larchmont, N.Y.) · 2024Article
- Microorganisms associated with hedgehog arthropods.Parasites & vectors · 2023Review
- Transposon mutagenesis of Rickettsia felis sca1 confers a distinct phenotype during flea infection.PLoS pathogens · 2022Article
- Review
- Culture Isolate ofInternational journal of environmental research and public health · 2022Article
- Manifestations and Management of Flea-Borne Rickettsioses.Research and reports in tropical medicine · 2021Review
- Molecular detection of Rickettsia felis in dogs, rodents and cat fleas in Zambia.Parasites & vectors · 2019Article
- Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines.Ticks and tick-borne diseases · 2019Article
- Spotted Fever GroupInfection and immunity · 2019Article
- A Tangled Web: Origins of Reproductive Parasitism.Genome biology and evolution · 2018Article
- Rickettsia felis: A Review of Transmission Mechanisms of an Emerging Pathogen.Tropical medicine and infectious disease · 2017Review
- Effect of Rickettsia felis Strain Variation on Infection, Transmission, and Fitness in the Cat Flea (Siphonaptera: Pulicidae).Journal of medical entomology · 2017Article
- Comparative vertical transmission of Rickettsia by Dermacentor variabilis and Amblyomma maculatum.Ticks and tick-borne diseases · 2017Article
- Flea-Borne Rickettsioses and Rickettsiae.The American journal of tropical medicine and hygiene · 2017Review
- Shell-vial culture and real-time PCR applied to Rickettsia typhi and Rickettsia felis detection.World journal of microbiology & biotechnology · 2015Article
- Cofeeding intra- and interspecific transmission of an emerging insect-borne rickettsial pathogen.Molecular ecology · 2015Article
- Morphological and molecular characterization of a sexually reproducing colony of the booklouse Liposcelis bostrychophila (Psocodea: Liposcelididae) found in Arizona.Scientific reports · 2015Article
- Ticks and rickettsial infection in the wildlife of two regions of the Brazilian Amazon.Experimental & applied acarology · 2015Article
- Candidatus 'Rickettsia senegalensis' in cat fleas in Senegal.New microbes and new infections · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Rickettsial diversity is intriguing in that some species are transmissible to vertebrates, while others appear exclusive to invertebrate hosts. Of particular interest is Rickettsia felis, identifiable in both stored product insect pests and hematophagous disease vectors. To understand rickettsial survival tactics in, and probable movement between, both insect systems will explicate the determinants of rickettsial pathogenicity. Towards this objective, a population of Liposcelis bostrychophila, common booklice, was successfully used for rickettsial isolation in ISE6 (tick-derived cells). Rickettsiae were also observed in L. bostrychophila by electron microscopy and in paraffin sections of booklice by immunofluorescence assay using anti-R. felis polyclonal antibody. The isolate, designated R. felis strain LSU-Lb, resembles typical rickettsiae when examined by microscopy. Sequence analysis of portions of the Rickettsia specific 17-kDa antigen gene, citrate synthase (gltA) gene, rickettsial outer membrane protein A (ompA) gene, and the presence of the R. felis plasmid in the cell culture isolate confirmed the isolate as R. felis. Variable nucleotide sequences from the isolate were obtained for R. felis-specific pRF-associated putative tldD/pmbA. Expression of rickettsial outer membrane protein B (OmpB) was verified in R. felis (LSU-Lb) using a monoclonal antibody. Additionally, a quantitative real-time PCR assay was used to identify a significantly greater median rickettsial load in the booklice, compared to cat flea hosts. With the potential to manipulate arthropod host biology and infect vertebrate hosts, the dual nature of R. felis provides an excellent model for the study of rickettsial pathogenesis and transmission. In addition, this study is the first isolation of a rickettsial pathogen from a non-hematophagous arthropod.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.