ArticleMolecular systems biology2011
Comprehensive quantitative analysis of central carbon and amino-acid metabolism in Saccharomyces cerevisiae under multiple conditions by targeted proteomics.
Article in Molecular systems biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Protein isoform-specific validation defines multiple chloride intracellular channel and tropomyosin isoforms as serological biomarkers of ovarian cancer.Journal of proteomics · 2013Trial
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- CFSA: Comparative flux sampling analysis as a guide for strain design.Metabolic engineering communications · 2024Article
- A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells.iScience · 2024Article
- Real-Time Assessment of Intracellular Metabolites in Single Cells through RNA-Based Sensors.Biomolecules · 2023Review
- Pea-Tea Intercropping Improves Tea Quality through Regulating Amino Acid Metabolism and Flavonoid Biosynthesis.Foods (Basel, Switzerland) · 2022Article
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- Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Energy metabolism controls phenotypes by protein efficiency and allocation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Layers of regulation of cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2018Article
- Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.Journal of proteomics · 2018Review
- TheG3 (Bethesda, Md.) · 2018Article
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- Evolutionary adaptations to new environments generally reverse plastic phenotypic changes.Nature communications · 2018Article
- Few regulatory metabolites coordinate expression of central metabolic genes in Escherichia coli.Molecular systems biology · 2017Article
- Prediction of Hopeless Peptides Unlikely to be Selected for Targeted Proteome Analysis.Mass spectrometry (Tokyo, Japan) · 2017Article
- Article
- Mass spectrometry-assisted gel-based proteomics in cancer biomarker discovery: approaches and application.Theranostics · 2017Review
- In silico metabolic network analysis of Arabidopsis leaves.BMC systems biology · 2016Article
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7 authors.
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Abstract
Decades of biochemical research have identified most of the enzymes that catalyze metabolic reactions in the yeast Saccharomyces cerevisiae. The adaptation of metabolism to changing nutritional conditions, in contrast, is much less well understood. As an important stepping stone toward such understanding, we exploit the power of proteomics assays based on selected reaction monitoring (SRM) mass spectrometry to quantify abundance changes of the 228 proteins that constitute the central carbon and amino-acid metabolic network in the yeast Saccharomyces cerevisiae, at five different metabolic steady states. Overall, 90% of the targeted proteins, including families of isoenzymes, were consistently detected and quantified in each sample, generating a proteomic data set that represents a nutritionally perturbed biological system at high reproducibility. The data set is near comprehensive because we detect 95-99% of all proteins that are required under a given condition. Interpreted through flux balance modeling, the data indicate that S. cerevisiae retains proteins not necessarily used in a particular environment. Further, the data suggest differential functionality for several metabolic isoenzymes.
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