ArticleProceedings of the National Academy of Sciences of the United States of America2011
Escherichia coli sister chromosome separation includes an abrupt global transition with concomitant release of late-splitting intersister snaps.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
72 citing papers in PubMed, 110 citations in OpenAlex.
- Spatio-temporal organization of theEcoSal Plus · 2024Review
- Compaction and Segregation of DNA inLife (Basel, Switzerland) · 2024Review
- Chromosome organization shapes replisome dynamics in Caulobacter crescentus.Nature communications · 2024Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli.Nature communications · 2023Article
- Creating Polyploid Escherichia Coli and Its Application in Efficient L-Threonine Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- The Quantification of Bacterial Cell Size: Discrepancies Arise from Varied Quantification Methods.Life (Basel, Switzerland) · 2023Article
- Article
- Escherichia coli cell factories with altered chromosomal replication scenarios exhibit accelerated growth and rapid biomass production.Microbial cell factories · 2022Article
- Psoralen mapping reveals a bacterial genome supercoiling landscape dominated by transcription.Nucleic acids research · 2022Article
- Coupling between DNA replication, segregation, and the onset of constriction in Escherichia coli.Cell reports · 2022Article
- Steric interactions and out-of-equilibrium processes control the internal organization of bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Nonrandom segregation of sister chromosomes byProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Nucleoid-mediated positioning and transport in bacteria.Current genetics · 2020Review
- Measuring transcription at a single gene copy reveals hidden drivers of bacterial individuality.Nature microbiology · 2019Article
- Watching DNA Replication Inhibitors in Action: Exploiting Time-Lapse Microfluidic Microscopy as a Tool for Target-Drug Interaction Studies inAntimicrobial agents and chemotherapy · 2019Article
- Article
- Segregation but Not Replication of themBio · 2018Article
- The Origin of Chromosomal Replication Is Asymmetrically Positioned on the Mycobacterial Nucleoid, and the Timing of Its Firing Depends on HupB.Journal of bacteriology · 2018Article
- Review
12 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The basis for segregation of sister chromosomes in bacteria is not established. We show here that two discrete ~150-kb regions, both located early in the right replichore, exhibit prolonged juxtaposition of sister loci, for 20 and 30 min, respectively, after replication. Flanking regions, meanwhile, separate. Thus, the two identified regions comprise specialized late-splitting intersister connections or snaps. Sister snap loci separate simultaneously in both snap regions, concomitant with a major global nucleoid reorganization that results in emergence of a bilobed nucleoid morphology. Split snap loci move rapidly apart to a separation distance comparable with one-half the length of the nucleoid. Concomitantly, at already split positions, sister loci undergo further separation to a comparable distance. The overall consequence of these and other effects is that thus far replicated sister chromosomes become spatially separated (individualized) into the two nucleoid lobes, while the terminus region (and likely, all unreplicated portions of the chromosome) moves to midcell. These and other findings imply that segregation of Escherichia coli sister chromosomes is not a smooth continuous process but involves at least one and likely, two major global transition(s). The presented patterns further suggest that accumulation of internal intranucleoid forces and constraining of these forces by snaps play central roles in global chromosome dynamics. They are consistent with and supportive of our previous proposals that individualization of sisters in E. coli is driven primarily by internally generated pushing forces and is directly analogous to sister individualization at the prophase to prometaphase transition of the eukaryotic cell cycle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.