ArticleProceedings of the National Academy of Sciences of the United States of America2011
Polarity proteins Bem1 and Cdc24 are components of the filamentous fungal NADPH oxidase complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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61 citing papers in PubMed, 136 citations in OpenAlex.
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- Cdc25-Mediated Activation of the Small GTPase RasB Is Essential for Hyphal Fusion and Symbiotic Infection of Epichloë festucae.Molecular plant pathology · 2026Article
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- Infection-specific transcriptional patterns of the maize pathogen Cochliobolus heterostrophus unravel genes involved in asexual development and virulence.Molecular plant pathology · 2024Article
- The transcription factor Ste12-like increases the mycelial abiotic stress tolerance and regulates the fruiting body development ofFrontiers in microbiology · 2023Article
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- Reactive Oxygen Species Distribution Involved in Stipe Gradient Elongation in the MushroomCells · 2022Article
- Transcriptomic Analysis Reveals That Rho GTPases Regulate Trap Development and Lifestyle Transition of the Nematode-Trapping FungusMicrobiology spectrum · 2022Article
- NADPH Oxidases Play a Role in PathogenicityFrontiers in cellular and infection microbiology · 2022Article
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- Molecular mechanisms in grass-Epichloë interactions: towards endophyte driven farming to improve plant fitness and immunity.World journal of microbiology & biotechnology · 2020Review
- Deep Evolutionary History of the Phox and Bem1 (PB1) Domain Across Eukaryotes.Scientific reports · 2020Article
- The NADPH oxidase in Volvariella volvacea and its differential expression in response to mycelial ageing and mechanical injury.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Review
- Reactive oxygen species: A generalist in regulating development and pathogenicity of phytopathogenic fungi.Computational and structural biotechnology journal · 2020Review
- Crosstalk Between Pheromone Signaling and NADPH Oxidase Complexes Coordinates Fungal Developmental Processes.Frontiers in microbiology · 2020Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulated synthesis of reactive oxygen species (ROS) by membrane-bound fungal NADPH oxidases (Nox) plays a key role in fungal morphogenesis, growth, and development. Generation of reactive oxygen species (ROS) by the plant symbiotic fungus, Epichloë festucae, requires functional assembly of a multisubunit complex composed of NoxA, a regulatory component, NoxR, and the small GTPase RacA. However, the mechanism for assembly and activation of this complex at the plasma membrane is unknown. We found by yeast two-hybrid and coimmunoprecipitation assays that E. festucae NoxR interacts with homologs of the yeast polarity proteins, Bem1 and Cdc24, and that the Phox and Bem1 (PB1) protein domains found in these proteins are essential for these interactions. GFP fusions of BemA, Cdc24, and NoxR preferentially localized to actively growing hyphal tips and to septa. These proteins interact with each other in vivo at these same cellular sites as shown by bimolecular fluorescent complementation assays. The PB1 domain of NoxR is essential for localization to the hyphal tip. An E. festucae ΔbemA mutant was defective in hyphal morphogenesis and growth in culture and in planta. The changes in fungal growth in planta resulted in a defective symbiotic interaction phenotype. Our inability to isolate a Δcdc24 mutant suggests this gene is essential. These results demonstrate that BemA and Cdc24 play a critical role in localizing NoxR protein to sites of fungal hyphal morphogenesis and growth. Our findings identify a potential shared ancestral link between the protein machinery required for fungal polarity establishment and the Nox complex controlling cellular differentiation.
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