ArticleDiabetes2011
Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism.
Article in Diabetes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Cavin gene family and caveolae-related disorders: pathogenetic roles and possible mechanisms.Cell communication and signaling : CCS · 2026Review
- Lessons from cavin-1 deficiency.Biochemical Society transactions · 2020Review
- Article
- PTRF acts as an adipokine contributing to adipocyte dysfunctionality and ectopic lipid deposition.Journal of physiology and biochemistry · 2018Article
- Dephosphorylated Polymerase I and Transcript Release Factor Prevents Allergic Asthma Exacerbations by Limiting IL-33 Release.Frontiers in immunology · 2018Article
- Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue.Molecular and cellular biology · 2017Article
- The Niemann-Pick C1 gene interacts with a high-fat diet to promote weight gain through differential regulation of central energy metabolism pathways.American journal of physiology. Endocrinology and metabolism · 2017Article
- PTRF/Cavin-1 promotes efficient ribosomal RNA transcription in response to metabolic challenges.eLife · 2016Article
- Studying mechanisms of cAMP and cyclic nucleotide phosphodiesterase signaling in Leydig cell function with phosphoproteomics.Cellular signalling · 2016Article
- A phosphoinositide-binding cluster in cavin1 acts as a molecular sensor for cavin1 degradation.Molecular biology of the cell · 2015Article
- Cavin-1 and Caveolin-1 are both required to support cell proliferation, migration and anchorage-independent cell growth in rhabdomyosarcoma.Laboratory investigation; a journal of technical methods and pathology · 2015Article
- Cavin family proteins and the assembly of caveolae.Journal of cell science · 2015Review
- Caveolin-1 expression and cavin stability regulate caveolae dynamics in adipocyte lipid store fluctuation.Diabetes · 2014Article
- Polymerase I and transcript release factor (PTRF) regulates adipocyte differentiation and determines adipose tissue expandability.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- The genetics of fat distribution.Diabetologia · 2014Review
- Pleiotropic effects of cavin-1 deficiency on lipid metabolism.The Journal of biological chemistry · 2014Article
- Article
- Caveola-forming proteins caveolin-1 and PTRF in prostate cancer.Nature reviews. Urology · 2013Review
- Caveolin-1 and polymerase I and transcript release factor: new players in insulin-like growth factor-I receptor signaling.Journal of endocrinological investigation · 2013Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectivePolymerase I and transcript release factor (PTRF) is a protein highly expressed in adipose tissue and is an integral structural component of caveolae. Here, we report on a novel role of PTRF in lipid mobilization. RESEARCH DESIGN AND
methodsPTRF expression was examined in different adipose depots of mice during fasting, refeeding, and after administration of catecholamines and insulin. Involvement of PTRF during lipolysis was studied upon PTRF knockdown and overexpression and mutation of PTRF phosphorylation sites in 3T3-L1 adipocytes.
resultsPTRF expression in mouse white adipose tissue (WAT) is regulated by nutritional status, increasing during fasting and decreasing to baseline after refeeding. Expression of PTRF also is hormonally regulated because treatment of mice with insulin leads to a decrease in expression, whereas isoproterenol increases expression in WAT. Manipulation of PTRF levels revealed a role of PTRF in lipolysis. Lentiviral-mediated knockdown of PTRF resulted in a marked attenuation of glycerol release in response to isoproterenol. Conversely, overexpressing PTRF enhanced isoproterenol-stimulated glycerol release. Mass-spectrometric analysis revealed that PTRF is phosphorylated at multiple sites in WAT. Mutation of serine 42, threonine 304, or serine 368 to alanine reduced isoproterenol-stimulated glycerol release in 3T3-L1 adipocytes.
conclusionsOur study is the first direct demonstration for a novel adipose tissue-specific function of PTRF as a mediator of lipolysis and also shows that phosphorylation of PTRF is required for efficient fat mobilization.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.