ArticleBiophysical journal2011
Fluctuation methods to study protein aggregation in live cells: concanavalin A oligomers formation.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?Membranes · 2025Review
- Transportan 10 Induces Perturbation and Pores Formation in Giant Plasma Membrane Vesicles Derived from Cancer Liver Cells.Biomolecules · 2023Article
- Peptide-Membrane Interactions Monitored by Fluorescence Lifetime Imaging: A Study Case of Transportan 10.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Article
- Using enhanced number and brightness to measure protein oligomerization dynamics in live cells.Nature protocols · 2019Article
- Assaying Homodimers of NF-κB in Live Single Cells.Frontiers in immunology · 2019Article
- State-of-the-Art Fluorescence Fluctuation-Based Spectroscopic Techniques for the Study of Protein Aggregation.International journal of molecular sciences · 2018Review
- Scanning number and brightness yields absolute protein concentrations in live cells: a crucial parameter controlling functional bio-molecular interaction networks.Biophysical reviews · 2018Review
- Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor.International journal of molecular sciences · 2017Article
- Article
- Number and brightness analysis of sFRP4 domains in live cells demonstrates vesicle association signal of the NLD domain and dynamic intracellular responses to Wnt3a.The international journal of biochemistry & cell biology · 2015Article
- Quantifying protein-protein interactions of peripheral membrane proteins by fluorescence brightness analysis.Biophysical journal · 2014Article
- Number and Brightness analysis of alpha-synuclein oligomerization and the associated mitochondrial morphology alterations in live cells.Biochimica et biophysica acta · 2014Article
- Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.PloS one · 2013Article
- An elegant way to quantitatively analyze oligomer formation in solution.Biophysical journal · 2012Article
- Regulation from within: the cytoskeleton in transmembrane signaling.Trends in cell biology · 2012Review
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Authors and funding
6 authors.
Funding
Abstract
Prefibrillar oligomers of proteins are suspected to be the primary pathogenic agents in several neurodegenerative diseases. A key approach for elucidating the pathogenic mechanisms is to probe the existence of oligomers directly in living cells. In this work, we were able to monitor the process of aggregation of Concanavalin A in live cells. We used number and brightness analysis, two-color cross number and brightness analysis, and Raster image correlation spectroscopy to obtain the number of molecules, aggregation state, and diffusion coefficient as a function of time and cell location. We observed that binding of Concanavalin A to the membrane and the formation of small aggregates paralleled cell morphology changes, indicating progressive cell compaction and death. Upon protein aggregation, we observed increased membrane water penetration as reported by Laurdan generalized polarization imaging.
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