Evidence map›Paper›PMID 21280010›Full record

ArticleArthritis and rheumatism2011

DEK in the synovium of patients with juvenile idiopathic arthritis: characterization of DEK antibodies and posttranslational modification of the DEK autoantigen.

Nirit Mor-Vaknin, Ferdinand Kappes, Amalie E Dick, Maureen Legendre, Catalina Damoc, Seagal Teitz-Tennenbaum, Roland Kwok, Elisa Ferrando-May, Barbara S Adams, David M Markovitz

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthritis and rheumatism, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 78 citations in OpenAlex.

  1. Trial
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Nirit Mor-VakninUniversity of Michigan, Ann Arbor, MI 48109-5640, USA. morvak@umich.edu
Ferdinand Kappes
Amalie E Dick
Maureen Legendre
Catalina Damoc
Seagal Teitz-Tennenbaum
Roland Kwok
Elisa Ferrando-May
Barbara S Adams
David M Markovitz
University of Michigan–Ann Arbor · USUniversity of Konstanz · DEUniversity of California, Davis · US

Funding

MICHIGAN INSTITUTE FOR CLINICAL AND HEALTH RESEARCH (MICHR) (UL1): (BPCA)UL1RR024986 · NCRR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SHANLEY, THOMAS P. · 2007 to 2011
$51.2M
Vector CoreP30AR048310 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MACOSKA, JILL A. · 2001 to 2010
$5.9M
CXC Chemokines and HIV PathogenesisR01AI062248 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MARKOVITZ, DAVID MICHAEL · 2005 to 2009
$1.6M
DEK and TNF inhibitors in juvenile arthritisR01AI087128 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MARKOVITZ, DAVID MICHAEL · 2009 to 2012
$1.5M
Coregulatory factor modulation of CREB activityR01DK067102 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KWOK, ROLAND P · 2006 to 2010
$1.3M
RESEARCH TRAINING IN TRANSLATIONAL TUMOR IMMUNOLOGYT32CA088784 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CHANG, ALFRED E · 2001 to 2005
$1.3M
The Role of DEK in the Development of ArthritisK01AR055620 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MOR-VAKNIN, NIRIT · 2009 to 2013
$541k
The Role of DEK in the Development of ArthritisR03AR056748 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MOR-VAKNIN, NIRIT · 2009 to 2011
$206k
NCI NIH HHS T32 CA088784NCI NIH HHS T32-CA-88784-03NCRR NIH HHS UL1 RR024986NCRR NIH HHS UL1-RR-024986NIAID NIH HHS R01 AI062248NIAID NIH HHS R01-AI-062248NIAID NIH HHS R01 AI087128NIAID NIH HHS R01-AI-087128NIAMS NIH HHS 5-P30-AR-048310-07NIAMS NIH HHS K01 AR055620NIAMS NIH HHS K01-AR-055620NIAMS NIH HHS P30 AR048310NIAMS NIH HHS R03 AR056748NIAMS NIH HHS R03-AR-056748-01NIDDK NIH HHS R01 DK067102NIDDK NIH HHS R01-DK-067102
6 · The paper itself

Abstract

objectiveDEK is a nuclear phosphoprotein and autoantigen in a subset of children with juvenile idiopathic arthritis (JIA). Autoantibodies to DEK are also found in a broad spectrum of disorders associated with abnormal immune activation. We previously demonstrated that DEK is secreted by macrophages, is released by apoptotic T cells, and attracts leukocytes. Since DEK has been identified in the synovial fluid (SF) of patients with JIA, this study was undertaken to investigate how DEK protein and/or autoantibodies may contribute to the pathogenesis of JIA.

methodsDEK autoantibodies, immune complexes (ICs), and synovial macrophages were purified from the SF of patients with JIA. DEK autoantibodies and ICs were purified by affinity-column chromatography and analyzed by 2-dimensional gel electrophoresis, immunoblotting, and enzyme-linked immunosorbent assay. DEK in supernatants and exosomes was purified by serial centrifugation and immunoprecipitation with magnetic beads, and posttranslational modifications of DEK were identified by nano-liquid chromatography tandem mass spectrometry (nano-LC-MS/MS).

resultsDEK autoantibodies and protein were found in the SF of patients with JIA. Secretion of DEK by synovial macrophages was observed both in a free form and via exosomes. DEK autoantibodies (IgG2) may activate the complement cascade, primarily recognize the C-terminal portion of DEK protein, and exhibit higher affinity for acetylated DEK. Consistent with these observations, DEK underwent acetylation on an unprecedented number of lysine residues, as demonstrated by nano-LC-MS/MS.

conclusionThese results indicate that DEK can contribute directly to joint inflammation in JIA by generating ICs through high-affinity interaction between DEK and DEK autoantibodies, a process enhanced by acetylation of DEK in the inflamed joint.

Indexed as

Protein Processing, Post-TranslationalAcetylationAntigen-Antibody ComplexArthritis, JuvenileAutoantibodiesAutoantigensChildChromosomal Proteins, Non-HistoneHumansJointsMacrophagesOncogene ProteinsPoly-ADP-Ribose Binding ProteinsSynovial FluidSynovial MembraneAntigen-Antibody ComplexAutoantibodiesAutoantigensChromosomal Proteins, Non-HistoneDEK protein, humanOncogene ProteinsPoly-ADP-Ribose Binding Proteins

Identifiers

PMID21280010
PMCPMC3117121
OpenAlexW1973182382

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.