ArticleArthritis and rheumatism2011
Systemic lupus erythematosus serum deposits C4d on red blood cells, decreases red blood cell membrane deformability, and promotes nitric oxide production.
Article in Arthritis and rheumatism, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- CR1-mediated ATP release by human red blood cells promotes CR1 clustering and modulates the immune transfer process.The Journal of biological chemistry · 2013Trial
- Scavenger receptors: key players in the immunological puzzle of lupus.Frontiers in lupus · 2025Article
- A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells.Analytical methods : advancing methods and applications · 2022Article
- RBC membrane biomechanics and Plasmodium falciparum invasion: probing beyond ligand-receptor interactions.Trends in parasitology · 2022Review
- Red blood cells in type 1 diabetes and multiple sclerosis and technologies to measure their emerging roles.Journal of translational autoimmunity · 2022Review
- Complement Deposition on the Surface of RBC After Trauma Serves a Biomarker of Moderate Trauma Severity: A Prospective Study.Shock (Augusta, Ga.) · 2020Article
- Cell-bound complement activation products in SLE.Lupus science & medicine · 2017Review
- Sickle cell disease biochip: a functional red blood cell adhesion assay for monitoring sickle cell disease.Translational research : the journal of laboratory and clinical medicine · 2016Article
- Ligation of Glycophorin A Generates Reactive Oxygen Species Leading to Decreased Red Blood Cell Function.PloS one · 2016Article
- Dynamic actin filaments control the mechanical behavior of the human red blood cell membrane.Molecular biology of the cell · 2015Article
- Bio-inspired cryo-ink preserves red blood cell phenotype and function during nanoliter vitrification.Advanced materials (Deerfield Beach, Fla.) · 2014Article
- An ultrastructural analysis of platelets, erythrocytes, white blood cells, and fibrin network in systemic lupus erythematosus.Rheumatology international · 2014Article
- Differential microRNA profile and post-transcriptional regulation exist in systemic lupus erythematosus patients with distinct autoantibody specificities.Journal of clinical immunology · 2014Article
- C4d deposits on the surface of RBCs in trauma patients and interferes with their function.Critical care medicine · 2014Article
- Cell-bound complement activation products in systemic lupus erythematosus: comparison with anti-double-stranded DNA and standard complement measurements.Lupus science & medicine · 2014Article
- Complement in the immunopathogenesis of rheumatic disease.Nature reviews. Rheumatology · 2012Review
- A model for lupus brain disease.Immunological reviews · 2012Review
- Induction of anti-Ro60/anti-La by immunisation with spectrin and induction of anti-spectrin by immunisation with Ro60 and 4-hydroxy-2-nonenal-modified Ro60 immunisation.Clinical and experimental rheumatologyArticle
- Probing the Effects of Ionizing Radiation on Young's Modulus of Human Erythrocytes Cytoskeleton using Atomic Force Microscopy.Journal of medical physicsArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveSystemic lupus erythematosus (SLE) is characterized by intravascular activation of the complement system and deposition of complement fragments (C3 and C4) on plasma membranes of circulating cells, including red blood cells (RBCs). The aim of this study was to address whether this process affects the biophysical properties of RBCs.
methodsSerum and RBCs were isolated from patients with SLE and healthy controls. RBCs from healthy universal donors (type O, Rh negative) were incubated with SLE or control serum. We used flow cytometry to assess complement fragment deposition on RBCs. RBC membrane deformability was measured using 2-dimensional microchannel arrays. Protein phosphorylation levels were quantified by Western blotting.
resultsIncubation of healthy universal donor RBCs with sera from patients with SLE, but not with control sera, led to deposition of C4d fragments on the RBCs. Complement-decorated RBCs exhibited significant decreases in both membrane deformability and flickering. Sera from SLE patients triggered a transitory Ca(++) influx in RBCs that was associated with decreased phosphorylation of β-spectrin and with increased phosphorylation of band 3, two key proteins of RBC cytoskeleton. Finally, incubation with SLE sera led to the production of nitric oxide by RBCs, whereas this did not occur with control sera.
conclusionOur data suggest that complement activation in patients with SLE leads to calcium-dependent cytosketeletal changes in RBCs that render them less deformable, probably impairing their flow through capillaries. This phenomenon may negatively affect the delivery of oxygen to the tissues.
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