Evidence map›Paper›PMID 21279639›Full record

ArticleEuropean biophysics journal : EBJ2011

Two methods of temperature control for single-molecule measurements.

Matthew A B Baker, Yuichi Inoue, Kuniaki Takeda, Akihiko Ishijima, Richard M Berry

Abstract read
PubMed Publisher
In one paragraph

Article in European biophysics journal : EBJ, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Review
  3. Probing DNA helicase kinetics with temperature-controlled magnetic tweezers.Small (Weinheim an der Bergstrasse, Germany) · 2015
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Matthew A B BakerClarendon Laboratory, Oxford University, UK.
Yuichi Inoue
Kuniaki Takeda
Akihiko Ishijima
Richard M Berry
Tohoku University · JPUniversity of Oxford · GB

Funding

Biotechnology and Biological Sciences Research Council BB/E00458X/1Biotechnology and Biological Sciences Research Council BB/H01991X/1
6 · The paper itself

Abstract

Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.

Indexed as

TemperatureAnimalsBacterial ProteinsCalibrationEscherichia coliFlagellaMicroscopyMyosin Type IIRabbitsTorqueBacterial ProteinsMyosin Type II

Identifiers

PMID21279639
OpenAlexW1989704911

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.