ArticleCurrent microbiology2011
Multiplex PCR assay for the detection of five putative virulence genes encoded in verotoxigenic Escherichia coli plasmids.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Shiga toxin-producing Escherichia coli plasmid diversity reveals virulence potential and control opportunities in animal hosts.Scientific reports · 2025Article
- Occurrence and genetic characterization of Shiga toxin-producing Escherichia coli on bovine and pork carcasses and the environment from transport trucks.World journal of microbiology & biotechnology · 2023Article
- Pathogenic and phylogenetic characteristics of non-O157 Shiga toxin-producingJournal of veterinary science · 2018Article
- An evaluation of the virulence and adherence properties of avian pathogenicOne health (Amsterdam, Netherlands) · 2017Article
- Variation in the Distribution of Putative Virulence and Colonization Factors in Shiga Toxin-ProducingFrontiers in cellular and infection microbiology · 2017Article
- Prevalence, virulence potential, and pulsed-field gel electrophoresis profiling of Shiga toxin-producingGut pathogens · 2017Article
- Virulence gene profiles and population genetic analysis for exploration of pathogenic serogroups of Shiga toxin-producing Escherichia coli.Journal of clinical microbiology · 2013Article
- Shiga toxin-producing Escherichia coli: factors involved in virulence and cattle colonization.Virulence · 2013Review
- Molecular characterization of Shiga toxin-producing Escherichia coli isolated from the environment of a dairy farm.Current microbiology · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim was to perform a pentavalent PCR assay for the detection of putative virulence genes encoded in VTEC plasmids, katP, espP, subA, stcE, and ehxA. The five-specific primer pairs used in the assay do not interfere with each other and generate amplification products of 914, 774, 556, 399, and 262 bp. It was selected at random 39 strains belonged to 20 serotypes in order to evaluate the multiplex in a wide variety of strains. The results of this study indicate that it is possible to perform simultaneous amplification and search for recognized plasmid-encoded virulence markers from different E. coli serotypes and apply this technique to the genetic characterization of E. coli strains isolated from reservoirs, foods or patients. This complementary technique is a useful tool to detect interstrain differences for epidemiological studies and to provide information that could be related to the risk of human infection.
Indexed as
Identifiers
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Registered trials
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