ArticleThe EMBO journal2011
Controlled and stochastic retention concentrates dynein at microtubule ends to keep endosomes on track.
Article in The EMBO journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 89 citations in OpenAlex.
- Article
- Temporal Patterns of Angular Displacement of Endosomes: Insights into Motor Protein Exchange Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The Herpes Simplex Virus pUL16 and pUL21 Proteins Prevent Capsids from Docking at Nuclear Pore Complexes.PLoS pathogens · 2023Article
- Optimizing microtubule arrangements for rapid cargo capture.Biophysical journal · 2021Article
- Linking transport and translation of mRNAs with endosomes and mitochondria.EMBO reports · 2021Review
- Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes.Journal of fungi (Basel, Switzerland) · 2021Article
- Membrane Traffic inJournal of fungi (Basel, Switzerland) · 2021Review
- Modelling the motion of organelles in an elongated cell via the coordination of heterogeneous drift-diffusion and long-range transport.The European physical journal. E, Soft matter · 2021Article
- Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2021Article
- Article
- Genetic dissection of neuropeptide cell biology at high and low activity in a defined sensory neuron.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cytoplasmic Transport Machinery of the SPF27 Homologue Num1 in Ustilago maydis.Scientific reports · 2018Article
- Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
- Spatial organization of organelles in fungi: Insights from mathematical modelling.Fungal genetics and biology : FG & B · 2017Review
- Article
- Cell Biology of Hyphal Growth.Microbiology spectrum · 2017Review
- Co-delivery of cell-wall-forming enzymes in the same vesicle for coordinated fungal cell wall formation.Nature microbiology · 2016Article
- Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells.Nature communications · 2016Article
- Peroxisomes, lipid droplets, and endoplasmic reticulum "hitchhike" on motile early endosomes.The Journal of cell biology · 2015Article
- Discovery of a vezatin-like protein for dynein-mediated early endosome transport.Molecular biology of the cell · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Bidirectional transport of early endosomes (EEs) involves microtubules (MTs) and associated motors. In fungi, the dynein/dynactin motor complex concentrates in a comet-like accumulation at MT plus-ends to receive kinesin-3-delivered EEs for retrograde transport. Here, we analyse the loading of endosomes onto dynein by combining live imaging of photoactivated endosomes and fluorescent dynein with mathematical modelling. Using nuclear pores as an internal calibration standard, we show that the dynein comet consists of ∼55 dynein motors. About half of the motors are slowly turned over (T(1/2): ∼98 s) and they are kept at the plus-ends by an active retention mechanism involving an interaction between dynactin and EB1. The other half is more dynamic (T(1/2): ∼10 s) and mathematical modelling suggests that they concentrate at MT ends because of stochastic motor behaviour. When the active retention is impaired by inhibitory peptides, dynein numbers in the comet are reduced to half and ∼10% of the EEs fall off the MT plus-ends. Thus, a combination of stochastic accumulation and active retention forms the dynein comet to ensure capturing of arriving organelles by retrograde motors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.