ArticleThe Journal of biological chemistry2011
Molecular recognition of the Tes LIM2-3 domains by the actin-related protein Arp7A.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Strained actin binding by the Prickle2 LIM domains and their regulation in the full-length protein.Molecular biology of the cell · 2026Article
- Article
- Biallelic mutations inLife medicine · 2023Article
- A novel homozygous mutation in ACTL7A leads to male infertility.Molecular genetics and genomics : MGG · 2023Article
- Testis-specific actin-like 7A (ACTL7A) is an indispensable protein for subacrosomal-associated F-actin formation, acrosomal anchoring, and male fertility.Molecular human reproduction · 2023Article
- Stress fiber strain recognition by the LIM protein testin is cryptic and mediated by RhoA.Molecular biology of the cell · 2021Article
- Article
- Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice.American journal of human genetics · 2021Article
- Disruption inScience advances · 2020Article
- A Burst of Genetic Innovation in Drosophila Actin-Related Proteins for Testis-Specific Function.Molecular biology and evolution · 2020Article
- TES functions as a Mena-dependent tumor suppressor in gastric cancer carcinogenesis and metastasis.Cancer communications (London, England) · 2019Article
- Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans.Scientific reports · 2017Article
- The PET and LIM1-2 domains of testin contribute to intramolecular and homodimeric interactions.PloS one · 2017Article
- Article
- Immune Infertility Should Be Positively Diagnosed Using an Accurate Method by Monitoring the Level of Anti-ACTL7a Antibody.Scientific reports · 2016Article
- TESTIN Induces Rapid Death and Suppresses Proliferation in Childhood B Acute Lymphoblastic Leukaemia Cells.PloS one · 2016Article
- The E3 Ubiquitin Ligase TRIM9 Is a Filopodia Off Switch Required for Netrin-Dependent Axon Guidance.Developmental cell · 2015Article
- Testin on Atherosclerosis in Rabbits.Chinese medical journal · 2015Article
- Susceptibility to Klebsiella pneumonaie infection in collaborative cross mice is a complex trait controlled by at least three loci acting at different time points.BMC genomics · 2014Article
- Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton.Developmental cell · 2014Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
Abstract
Actin-related proteins (Arps) are a highly conserved family of proteins that have extensive sequence and structural similarity to actin. All characterized Arps are components of large multimeric complexes associated with chromatin or the cytoskeleton. In addition, the human genome encodes five conserved but largely uncharacterized "orphan" Arps, which appear to be mostly testis-specific. Here we show that Arp7A, which has 43% sequence identity with β-actin, forms a complex with the cytoskeletal proteins Tes and Mena in the subacrosomal layer of round spermatids. The N-terminal 65-residue extension to the actin-like fold of Arp7A interacts directly with Tes. The crystal structure of the 1-65(Arp7A)·LIM2-3(Tes)·EVH1(Mena) complex reveals that residues 28-49 of Arp7A contact the LIM2-3 domains of Tes. Two alanine residues from Arp7A that occupy equivalent apolar pockets in both LIM domains as well as an intervening GPAK linker that binds the LIM2-3 junction are critical for the Arp7A-Tes interaction. Equivalent occupied apolar pockets are also seen in the tandem LIM domain structures of LMO4 and Lhx3 bound to unrelated ligands. Our results indicate that apolar pocket interactions are a common feature of tandem LIM domain interactions, but ligand specificity is principally determined by the linker sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.