ArticlePlant physiology2011
Protein storage vacuoles are transformed into lytic vacuoles in root meristematic cells of germinating seedlings by multiple, cell type-specific mechanisms.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 91 citations in OpenAlex.
- Structural Features of Root Cells ofInternational journal of molecular sciences · 2026Article
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- Peptide AEDL Activates Metabolism and Autophagy in Root Cells ofInternational journal of molecular sciences · 2025Article
- Seeing is believing: Whole-cell electron tomography models of vacuole morphology and formation in the early-stage root cortex of Arabidopsis.The Plant cell · 2025Article
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling.Nature communications · 2023Article
- Functional Association between Storage Protein Mobilization and Redox Signaling in Narrow-Leafed Lupin (Genes · 2023Article
- Ab-GALFA, A bioassay for insect gall formation using the model plant Arabidopsis thaliana.Scientific reports · 2023Article
- Chloroplast Envelopes Play a Role in the Formation of Autophagy-Related Structures in Plants.Plants (Basel, Switzerland) · 2023Article
- Review
- Correlation of vacuole morphology with stomatal lineage development by whole-cell electron tomography.Plant physiology · 2022Article
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- Intracellular wetting mediates contacts between liquid compartments and membrane-bound organelles.The Journal of cell biology · 2021Article
- Multiple functions of the vacuole in plant growth and fruit quality.Molecular horticulture · 2021Review
- Bright Fluorescent Vacuolar Marker Lines Allow Vacuolar Tracing Across Multiple Tissues and Stress Conditions in Rice.International journal of molecular sciences · 2020Article
- Peptide transporter2 (PTR2) enhances water uptake during early seed germination in Arabidopsis thaliana.Plant molecular biology · 2020Article
- Phosphorylation of TIP3 Aquaporins duringCells · 2019Article
- A Review of Plant Vacuoles: Formation, Located Proteins, and Functions.Plants (Basel, Switzerland) · 2019Review
- Aquaporins influence seed dormancy and germination in response to stress.Plant, cell & environment · 2019Article
- Phosphoinositides control the localization of HOPS subunit VPS41, which together with VPS33 mediates vacuole fusion in plants.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
We have investigated the structural events associated with vacuole biogenesis in root tip cells of tobacco (Nicotiana tabacum) seedlings preserved by high-pressure freezing and freeze-substitution techniques. Our micrographs demonstrate that the lytic vacuoles (LVs) of root tip cells are derived from protein storage vacuoles (PSVs) by cell type-specific sets of transformation events. Analysis of the vacuole transformation pathways has been aided by the phytin-dependent black osmium staining of PSV luminal contents. In epidermal and outer cortex cells, the central LVs are formed by a process involving PSV fusion, storage protein degradation, and the gradual replacement of the PSV marker protein α-tonoplast intrinsic protein (TIP) with the LV marker protein γ-TIP. In contrast, in the inner cortex and vascular cylinder cells, the transformation events are more complex. During mobilization of the stored molecules, the PSV membranes collapse osmotically upon themselves, thereby squeezing the vacuolar contents into the remaining bulging vacuolar regions. The collapsed PSV membranes then differentiate into two domains: (1) vacuole "reinflation" domains that produce pre-LVs, and (2) multilamellar autophagosomal domains that are later engulfed by the pre-LVs. The multilamellar autophagosomal domains appear to originate from concentric sheets of PSV membranes that create compartments within which the cytoplasm begins to break down. Engulfment of the multilamellar autophagic vacuoles by the pre-LVs gives rise to the mature LVs. During pre-LV formation, the PSV marker α-TIP disappears and is replaced by the LV marker γ-TIP. These findings demonstrate that the central LVs of root cells arise from PSVs via cell type-specific transformation pathways.
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