ArticleJournal of bacteriology2011
The second RNA chaperone, Hfq2, is also required for survival under stress and full virulence of Burkholderia cenocepacia J2315.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- The Roles of TwoThe plant pathology journal · 2018Article
- Arginine Patch Predicts the RNA Annealing Activity of Hfq from Gram-Negative and Gram-Positive Bacteria.Journal of molecular biology · 2016Article
- Hfqs in Bacillus anthracis: Role of protein sequence variation in the structure and function of proteins in the Hfq family.Protein science : a publication of the Protein Society · 2015Article
- Hfq regulates antibacterial antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11.Microbial biotechnology · 2015Article
- Regulation of Hfq mRNA and protein levels in Escherichia coli and Pseudomonas aeruginosa by the Burkholderia cenocepacia MtvR sRNA.PloS one · 2014Article
- Small RNA-mediated regulation of host-pathogen interactions.Virulence · 2013Review
- MtvR is a global small noncoding regulatory RNA in Burkholderia cenocepacia.Journal of bacteriology · 2013Article
- Biochemical and functional studies on the Burkholderia cepacia complex bceN gene, encoding a GDP-D-mannose 4,6-dehydratase.PloS one · 2013Article
- The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.PloS one · 2012Article
- Regulation by small RNAs in bacteria: expanding frontiers.Molecular cell · 2011Review
- Hfq and its constellation of RNA.Nature reviews. Microbiology · 2011Review
- Small Regulatory RNA and Legionella pneumophila.Frontiers in microbiology · 2011Article
Corrections and comments
- Retraction · 2014-11-01Duplication of/in Image · Error in Image ·
- Retracted
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia cenocepacia J2315 is a highly virulent and epidemic clinical isolate of the B. cepacia complex (Bcc), a group of bacteria that have emerged as important pathogens to cystic fibrosis patients. This bacterium, together with all Bcc strains and a few other prokaryotes, is unusual for encoding in its genome two distinct and functional Hfq-like proteins. In this work, we show results indicating that the 188-amino-acid Hfq2 protein is required for the full virulence and stress resistance of B. cenocepacia J2315, despite the presence on its genome of the functional 79-amino-acid Hfq protein encoded by the hfq gene. Similar to other Hfq proteins, Hfq2 is able to bind RNA. However, Hfq2 is unique in its ability to apparently form trimers in vitro. Maximal transcription of hfq was observed in B. cenocepacia J2315 cells in the early exponential phase of growth. In contrast, hfq2 transcription reached maximal levels in cells in the stationary phase, depending on the CepR quorum-sensing regulator. These results suggest that tight regulation of the expression of these two RNA chaperones is required to maximize the fitness and virulence of this bacterium. In addition, the ability of Hfq2 to bind DNA, not observed for Hfq, suggests that Hfq2 might play additional roles besides acting as an RNA chaperone.
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