Evidence map›Paper›PMID 21278291›Full record

ArticleJournal of bacteriology2011

Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.

Sabine E Eckert, Francis Dziva, Roy R Chaudhuri, Gemma C Langridge, Daniel J Turner, Derek J Pickard, Duncan J Maskell, Nicholas R Thomson, Mark P Stevens

Abstract read
In one paragraph

Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sabine E EckertRoslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Bush Farm Road, Roslin, Midlothian EH25 9RG, United Kingdom.
Francis Dziva
Roy R Chaudhuri
Gemma C Langridge
Daniel J Turner
Derek J Pickard
Duncan J Maskell
Nicholas R Thomson
Mark P Stevens

Funding

Biotechnology and Biological Sciences Research Council BB/D017947/1Biotechnology and Biological Sciences Research Council BBS/E/I/00000715Biotechnology and Biological Sciences Research Council D017556Biotechnology and Biological Sciences Research Council D017947Wellcome Trust
6 · The paper itself

Abstract

Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.

Indexed as

AnimalsCattleCattle DiseasesChromosomes, BacterialDNA, BacterialDNA Transposable ElementsEscherichia coli O157Genetic FitnessGenotypeMutagenesis, InsertionalPlasmidsDNA, BacterialDNA Transposable Elements

Identifiers

PMID21278291
PMCPMC3067669

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.