ArticleJournal of bacteriology2011
The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Uptake of environmental DNA in Bacillus subtilis occurs all over the cell surface through a dynamic pilus structure.PLoS genetics · 2023Article
- Visualizing dynamic competence pili and DNA capture throughout the long axis ofJournal of bacteriology · 2023Article
- Visualizing dynamic competence pili and DNA capture throughout the long axis ofbioRxiv : the preprint server for biology · 2023Article
- Protein complexes in cells by AI-assisted structural proteomics.Molecular systems biology · 2023Article
- Single-Molecule Dynamics of DNA Receptor ComEA, Membrane Permease ComEC, and Taken-Up DNA in Competent Bacillus subtilis Cells.Journal of bacteriology · 2022Article
- "Take It or Leave It"-Factors Regulating Competence Development and DNA Uptake inInternational journal of molecular sciences · 2021Article
- Article
- The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis.Journal of bacteriology · 2020Review
- Super-Resolution Microscopy and Single-Molecule Tracking Reveal Distinct Adaptive Dynamics of MreB and of Cell Wall-Synthesis Enzymes.Frontiers in microbiology · 2020Article
- Article
- The Enigmatic Genome of an Obligate Ancient Spiroplasma Symbiont in a Hadal Holothurian.Applied and environmental microbiology · 2018Article
- The Bacillus BioBrick Box 2.0: expanding the genetic toolbox for the standardized work with Bacillus subtilis.Scientific reports · 2017Article
- Polyvalent Proteins, a Pervasive Theme in the Intergenomic Biological Conflicts of Bacteriophages and Conjugative Elements.Journal of bacteriology · 2017Article
- Structural characterization of the late competence protein ComFB from Bacillus subtilis.Bioscience reports · 2015Article
- Novel Flp pilus biogenesis-dependent natural transformation.Frontiers in microbiology · 2015Article
- Article
- Concerted spatio-temporal dynamics of imported DNA and ComE DNA uptake protein during gonococcal transformation.PLoS pathogens · 2014Article
- ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholerae cells.PLoS genetics · 2014Article
- Article
- DNA-uptake machinery of naturally competent Vibrio cholerae.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many bacteria possess the ability to actively take up DNA from the environment and incorporate it into the chromosome. RecA protein is the key protein achieving homologous recombination. Several of the proteins involved in the transport of DNA across the cell envelope assemble at a single or both cell poles in competent Bacillus subtilis cells. We show that the presumed structure that transports DNA across the cell wall, the pseudopilus, also assembles at a single or both cell poles, while the membrane receptor, ComEA, forms a mobile layer throughout the cell membrane. All other known Com proteins, including the membrane permease, localize again to the cell pole, revealing that the uptake machinery has three distinct layers. In cells having two uptake machineries, one complex is occasionally mobile, with pairs of proteins moving together, suggesting that a complete complex may lose anchoring and become mobile. Overall, the cell pole provides stable anchoring. Only one of two uptake machineries assembles RecA protein, suggesting that only one is competent for DNA transfer. FRAP (fluorescence recovery after photobleaching) analyses show that in contrast to known multiprotein complexes, the DNA uptake machinery forms a highly stable complex, showing little or no exchange with unbound molecules. When cells are converted into round spheroplasts, the structure persists, revealing that the assembly is highly stable and does not require the cell pole for its maintenance. High stability may be important to fulfill the mechanical function in pulling DNA across two cell layers.
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