ArticleJournal of bacteriology2011
Regulatory control of the Escherichia coli O157:H7 lpf1 operon by H-NS and Ler.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Breaking the silence-role of MucR as a virulence determinant inJournal of bacteriology · 2025Review
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- Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer's patches interactions.Scientific reports · 2017Article
- Article
- Finding Regulators Associated with the Expression of the Long Polar Fimbriae in Enteropathogenic Escherichia coli.Journal of bacteriology · 2015Article
- Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7.FEMS microbiology letters · 2014Article
- DNA looping-dependent autorepression of LEE1 P1 promoters by Ler in enteropathogenic Escherichia coli (EPEC).Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- H-NST induces LEE expression and the formation of attaching and effacing lesions in enterohemorrhagic Escherichia coli.PloS one · 2014Article
- Long polar fimbriae participates in the induction of neutrophils transepithelial migration across intestinal cells infected with enterohemorrhagic E. coli O157:H7.Frontiers in cellular and infection microbiology · 2014Article
- Ethanolamine and choline promote expression of putative and characterized fimbriae in enterohemorrhagic Escherichia coli O157:H7.Infection and immunity · 2014Article
- A distinct regulatory sequence is essential for the expression of a subset of nle genes in attaching and effacing Escherichia coli.Journal of bacteriology · 2012Article
- A double, long polar fimbria mutant of Escherichia coli O157:H7 expresses Curli and exhibits reduced in vivo colonization.Infection and immunity · 2012Article
- Molecular mechanisms that mediate colonization of Shiga toxin-producing Escherichia coli strains.Infection and immunity · 2012Review
- Indirect DNA readout by an H-NS related protein: structure of the DNA complex of the C-terminal domain of Ler.PLoS pathogens · 2011Article
- Review
- EHEC Adhesins.Microbiology spectrumArticle
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Long polar fimbriae 1 (Lpf1) of Escherichia coli O157:H7 is a tightly regulated adhesin, with H-NS silencing the transcriptional expression of the lpf1 operon while Ler (locus of enterocyte effacement-encoded regulator) acts as an antisilencer. We mapped the minimal regulatory region of lpf1 required for H-NS- and Ler-mediated regulation and found that it is 79% AT rich. Three putative sites for H-NS binding were identified. Two of them, named silencer regulatory sequence 1 (SRS1) and SRS2, are located on a region that covers both of the lpf1 promoters (P1 and P2). The third putative H-NS binding site is located within the lpfA1 gene in a region extending from +258 bp to +545 bp downstream of ATG; however, this site does not seem to play a role in lpfA1 regulation under the conditions tested in this work. Ler was also found to interact with Ler binding sites (LBSs). Ler binding site 1 (LBS1) and LBS2 are located upstream of the two promoters. LBS1 overlaps SRS1, while LBS3 overlaps the P1 promoter and SRS2. Based on the experimental data, we propose that H-NS silences lpf1 expression by binding to both of the SRSs on the promoter region, forming an SRS-H-NS complex that prevents RNA polymerase-mediated transcription. A model of the regulation of the lpfA1 operon of E. coli O157:H7 by H-NS and Ler is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.