ArticleApplied and environmental microbiology2011
Poly-alpha-glutamic acid synthesis using a novel catalytic activity of RimK from Escherichia coli K-12.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 47 citations in OpenAlex.
- A posttranslational modification of fimbriae drives pathogenicity inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Preparation of Polylactic Acid/Modified Natto Composite Films and Property Evaluation.ACS omega · 2026Article
- A novel post-translational modification of fimbriae drives pathogenicity inbioRxiv : the preprint server for biology · 2025Article
- Molecular and structural characterization of aMicrobiology spectrum · 2025Article
- Secreted retropepsin-like enzymes are essential for stress tolerance and biofilm formation inmBio · 2025Article
- Identification of a novel lymphangiogenesis signature associated with immune cell infiltration in colorectal cancer based on bioinformatics analysis.BMC medical genomics · 2024Article
- Synthesis of Poly-γ-Glutamic Acid and Its Application in Biomedical Materials.Materials (Basel, Switzerland) · 2023Review
- Electrostatic Ratchet for Successive Peptide Synthesis in Nonribosomal Molecular Machine RimK.Journal of the American Chemical Society · 2023Article
- Large-scale phylogenomics of aquatic bacteria reveal molecular mechanisms for adaptation to salinity.Science advances · 2023Article
- Structural insights into the mechanism of adaptive ribosomal modification by Pseudomonas RimK.Proteins · 2023Article
- The Prognostic Significance of RIMKLB and Related Immune Infiltrates in Colorectal Cancers.Frontiers in genetics · 2022Article
- Systematic analysis of lysine malonylation inFrontiers in cellular and infection microbiology · 2022Article
- Rimklb mutation causes male infertility in mice.Scientific reports · 2021Article
- The Multiple Regulatory Relationship Between RNA-Chaperone Hfq and the Second Messenger c-di-GMP.Frontiers in microbiology · 2021Review
- Characterization of a membrane enzymatic complex for heterologous production of poly-γ-glutamate inMetabolic engineering communications · 2020Article
- Isolation and characterization of an exopolymer produced by Bacillus licheniformis: In vitro antiviral activity against enveloped viruses.Carbohydrate polymers · 2020Article
- Diamine Biosynthesis: Research Progress and Application Prospects.Applied and environmental microbiology · 2020Review
- Control of mRNA translation by dynamic ribosome modification.PLoS genetics · 2020Article
- Strategy for the Biosynthesis of Short Oligopeptides: Green and Sustainable Chemistry.Biomolecules · 2019Review
- Structural polymorphism of the Escherichia coli poly-α-L-glutamate synthetase RimK.Acta crystallographica. Section F, Structural biology communications · 2018Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly-L-α-amino acids have various applications because of their biodegradable properties and biocompatibility. Microorganisms contain several enzymes that catalyze the polymerization of L-amino acids in an ATP-dependent manner, but the products from these reactions contain amide linkages at the side residues of amino acids: e.g., poly-γ-glutamic acid, poly-ε-lysine, and cyanophycin. In this study, we found a novel catalytic activity of RimK, a ribosomal protein S6-modifying enzyme derived from Escherichia coli K-12. This enzyme catalyzed poly-α-glutamic acid synthesis from unprotected L-glutamic acid (Glu) by hydrolyzing ATP to ADP and phosphate. RimK synthesized poly-α-glutamic acid of various lengths; matrix-assisted laser desorption ionization-time of flight-mass spectrometry showed that a 46-mer of Glu (maximum length) was synthesized at pH 9. Interestingly, the lengths of polymers changed with changing pH. RimK also exhibited 86% activity after incubation at 55°C for 15 min, thus showing thermal stability. Furthermore, peptide elongation seemed to be catalyzed at the C terminus in a stepwise manner. Although RimK showed strict substrate specificity toward Glu, it also used, to a small extent, other amino acids as C-terminal substrates and synthesized heteropeptides. In addition, RimK-catalyzed modification of ribosomal protein S6 was confirmed. The number of Glu residues added to the protein varied with pH and was largest at pH 9.5.
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