ArticleApplied and environmental microbiology2011
Novel virulence gene and clustered regularly interspaced short palindromic repeat (CRISPR) multilocus sequence typing scheme for subtyping of the major serovars of Salmonella enterica subsp. enterica.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
61 citing papers in PubMed.
- Whole-genome insights into a multidrug-resistant, biofilm-forming, and pathogenic Salmonella Umbilo isolate and molecular characterization of rare Salmonella serovars from poultry.Poultry science · 2026Article
- CRISPR and CRISPR-MVLST reveal conserved spacer distribution and high similarity among Salmonella enterica serovar Infantis genomes from Brazil and other countries.Molecular genetics and genomics : MGG · 2024Article
- Drug resistance and virulence-associated genes screening in Salmonella enterica isolated from Caspian pony, Iran.Veterinary research forum : an international quarterly journal · 2024Review
- FromACS omega · 2023Review
- Combined Quantification and Deep Serotyping for Salmonella Risk Profiling in Broiler Flocks.Applied and environmental microbiology · 2023Article
- Advances in CRISPR/Cas-Based Strategies on Zoonosis.Transboundary and emerging diseases · 2023Review
- Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella.Microbiology spectrum · 2022Article
- Correlation between CRISPR Loci Diversity in Three Enterobacterial Taxa.International journal of molecular sciences · 2022Article
- The CRISPR genotypes and genetic diversity of different serogroups of nontyphoidal Salmonella in Guizhou Province, 2013-2018.PloS one · 2022Article
- Evaluation of the use of CRISPR loci for discrimination ofAIMS microbiology · 2022Article
- Comparison of Conventional Molecular and Whole-Genome Sequencing Methods for DifferentiatingMicroorganisms · 2021Article
- Article
- Will CRISPR-Cas9 Have Cards to Play Against Cancer? An Update on its Applications.Molecular biotechnology · 2021Review
- Antimicrobial Resistance and CRISPR Typing AmongFrontiers in microbiology · 2021Article
- Whole-Genome-Based Survey for Polyphyletic Serovars ofInternational journal of molecular sciences · 2020Article
- High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Utilization of Clustered Regularly Interspaced Short Palindromic Repeats to GenotypeFrontiers in microbiology · 2020Article
- Genomic Features of High-Priority Salmonella enterica Serovars Circulating in the Food Production Chain, Brazil, 2000-2016.Scientific reports · 2019Article
- Mechanisms of Type I-E and I-F CRISPR-Cas Systems inEcoSal Plus · 2019Review
- Persistent Infection and Long-Term Carriage of Typhoidal and Nontyphoidal Salmonellae.Clinical microbiology reviews · 2019Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica subsp. enterica is the leading cause of bacterial food-borne disease in the United States. Molecular subtyping methods are powerful tools for tracking the farm-to-fork spread of food-borne pathogens during outbreaks. In order to develop a novel multilocus sequence typing (MLST) scheme for subtyping the major serovars of S. enterica subsp. enterica, the virulence genes sseL and fimH and clustered regularly interspaced short palindromic repeat (CRISPR) loci were sequenced from 171 clinical isolates from nine Salmonella serovars, Salmonella serovars Typhimurium, Enteritidis, Newport, Heidelberg, Javiana, I 4,[5],12:i:-, Montevideo, Muenchen, and Saintpaul. The MLST scheme using only virulence genes was congruent with serotyping and identified epidemic clones but could not differentiate outbreaks. The addition of CRISPR sequences dramatically improved discriminatory power by differentiating individual outbreak strains/clones. Of particular note, the present MLST scheme provided better discrimination of Salmonella serovar Enteritidis strains than pulsed-field gel electrophoresis (PFGE). This method showed high epidemiologic concordance for all serovars screened except for Salmonella serovar Muenchen. In conclusion, the novel MLST scheme described in the present study accurately differentiated outbreak strains/clones of the major serovars of Salmonella, and therefore, it shows promise for subtyping this important food-borne pathogen during investigations of outbreaks.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.