ArticleMolecular and biochemical parasitology2011
Toxoplasma gondii toxolysin 4 is an extensively processed putative metalloproteinase secreted from micronemes.
Article in Molecular and biochemical parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Cryptosporidium parvum protease INS6 plays an important role in parasite proliferation and pathogenicity.PLoS neglected tropical diseases · 2025Article
- Protein characterization of the IgM triplet involved in the diagnosis of congenital toxoplasmosis.Parasite (Paris, France) · 2025Article
- Involvement of INS15 in the development and pathogenicity of the zoonotic pathogen Cryptosporidium parvum.PLoS neglected tropical diseases · 2024Article
- Article
- Article
- Characterization of Dense Granule Metalloproteinase INS-16 inInternational journal of molecular sciences · 2022Article
- Comparative Study of Two Insulinlike Proteases inMicroorganisms · 2021Article
- Insulinase-like Protease 1 Contributes to Macrogamont Formation in Cryptosporidium parvum.mBio · 2021Article
- Preliminary Characterization of Two Small Insulinase-Like Proteases inFrontiers in microbiology · 2021Article
- Genetic basis for virulence differences of various Cryptosporidium parvum carcinogenic isolates.Scientific reports · 2020Article
- Expression and Functional Studies of INS-5, an Insulinase-Like Protein inFrontiers in microbiology · 2020Article
- Characterization of INS-15, A Metalloprotease Potentially Involved in the Invasion ofMicroorganisms · 2019Article
- Article
- Characterization of a Species-Specific Insulinase-Like Protease inFrontiers in microbiology · 2019Article
- Characterization of metalloproteases and serine proteases of Toxoplasma gondii tachyzoites and their effect on epithelial cells.Parasitology research · 2019Article
- Metallopeptidases of Toxoplasma gondii: in silico identification and gene expression.Parasite (Paris, France) · 2018Article
- Article
- Identification of a TNF-α inducer MIC3 originating from the microneme of non-cystogenic, virulent Toxoplasma gondii.Scientific reports · 2016Article
- Research advances in microneme protein 3 of Toxoplasma gondii.Parasites & vectors · 2015Review
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2 authors at 1 institution in 1 country.
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Abstract
Proteases play central roles in cell invasion by Toxoplasma gondii and other apicomplexan parasites. Herein we report the cloning and characterization of a novel secretory putative metalloproteinase, Toxolysin 4 (TLN4). T. gondii tachyzoites store TLN4 in the micronemes and secrete it in response to elevated calcium, suggesting a possible role in cell invasion. TLN4 is initially synthesized as a large (∼260 kDa) precursor, which is extensively processed into multiple proteolytic fragments within the parasite secretory system. At least some of these proteolytic fragments remain associated in a large molecular complex. Whereas precomplementation with the TLN4 cDNA allowed disruption of the TLN4 gene, multiple attempts to directly knockout TLN4 without precomplementation failed. TLN4 knockout parasites were detected by PCR in transfected populations but were lost from the cultures during drug selection and growth suggesting that TLN4 contributes to parasite fitness.
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