ArticleGastroenterology2011
Activation of corticotropin-releasing factor receptor 2 mediates the colonic motor coping response to acute stress in rodents.
Article in Gastroenterology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Pooled it
- PVNAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Corticotropin-releasing factor type 1 receptors in the rat nodose ganglion are involved in the transduction of stress-induced visceral sensory signals to the brain.Journal of neuroendocrinology · 2025Article
- Otilonium Bromide Prevents Cholinergic Changes in the Distal Colon Induced by Chronic Water Avoidance Stress, a Rat Model of Irritable Bowel Syndrome.International journal of molecular sciences · 2023Article
- Psychological stress induces an increase in cholinergic enteric neuromuscular pathways mediated by glucocorticoid receptors.Frontiers in neuroscience · 2023Article
- Article
- Otilonium Bromide treatment prevents nitrergic functional and morphological changes caused by chronic stress in the distal colon of a rat IBS model.Journal of cellular and molecular medicine · 2021Article
- Intranasal Administration of Oxytocin Attenuates Stress Responses Following Chronic Complicated Stress in Rats.Journal of neurogastroenterology and motility · 2019Article
- VIP is involved in peripheral CRF-induced stimulation of propulsive colonic motor function and diarrhea in male rats.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- Brain and Gut CRF Signaling: Biological Actions and Role in the Gastrointestinal Tract.Current molecular pharmacology · 2018Review
- Gastric corticotropin-releasing factor influences mast cell infiltration in a rat model of functional dyspepsia.PloS one · 2018Article
- Frontline Science: Corticotropin-releasing factor receptor subtype 1 is a critical modulator of mast cell degranulation and stress-induced pathophysiology.Journal of leukocyte biology · 2017Article
- Endocrinology and the brain: corticotropin-releasing hormone signaling.Endocrine connections · 2017Review
- The Effect of Peripheral CRF Peptide and Water Avoidance Stress on Colonic and Gastric Transit in Guinea Pigs.Yonsei medical journal · 2017Article
- Corticotropin-releasing factor stimulates colonic motilityWorld journal of gastroenterology · 2017Article
- Repeated otilonium bromide administration prevents neurotransmitter changes in colon of rats underwent to wrap restraint stress.Journal of cellular and molecular medicine · 2017Article
- Lipopolysaccharide induces visceral hypersensitivity: role of interleukin-1, interleukin-6, and peripheral corticotropin-releasing factor in rats.Journal of gastroenterology · 2017Article
- Article
- Neonatal maternal separation increases susceptibility to experimental colitis and acute stress exposure in male mice.IBRO reports · 2016Article
- Sex- and corticotropin-releasing factor receptor 2- dependent actions of urocortin 1 during inflammation.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
BACKGROUND &
aimsCorticotropin-releasing factor receptor-1 (CRF(1)) mediates the stress-induced colonic motor activity. Less is known about the role of CRF(2) in the colonic response to stress.
methodsWe studied colonic contractile activity in rats and CRF(2)-/-, CRF-overexpressing, and wild-type mice using still manometry; we analyzed defecation induced by acute partial-restraint stress (PRS), and/or intraperitoneal injection of CRF ligands. In rats, we monitored activation of the colonic longitudinal muscle myenteric plexus (LMMP) neurons and localization of CRF(1) and CRF(2) using immunohistochemical and immunoblot analyses. We measured phosphorylation of extracellular signal-regulated kinase 1/2 by CRF ligands in primary cultures of LMMP neurons (PC-LMMPn) and cyclic adenosine monophosphate (cAMP) production in human embryonic kidney-293 cells transfected with CRF(1) and/or CRF(2).
resultsIn rats, a selective agonist of CRF(2) (urocortin 2) reduced CRF-induced defecation (>50%), colonic contractile activity, and Fos expression in the colonic LMMP. A selective antagonist of CRF(2) (astressin(2)-B) increased these responses. Urocortin 2 reduced PRS-induced colonic contractile activity in wild-type and CRF-overexpressing mice, whereas disruption of CRF(2) increased PRS-induced colonic contractile activity and CRF-induced defecation. CRF(2) colocalized with CRF(1) and neuronal nitric oxide synthase in the rat colon, LMMP, and PC-LMMPn. CRF-induced phosphorylation of extracellular signal-regulated kinase in PC-LMMPn; this was inhibited or increased by a selective antagonist of CRF(1) (NBI35965) or astressin(2)-B, respectively. The half maximal effective concentration, EC(50), for the CRF-induced cAMP response was 8.6 nmol/L in human embryonic kidney-293 cells that express only CRF(1); this response was suppressed 10-fold in cells that express CRF(1) and CRF(2).
conclusionsIn colon tissues of rodents, CRF(2) activation inhibits CRF(1) signaling in myenteric neurons and the stress-induced colonic motor responses. Disruption of CRF(2) function impairs colonic coping responses to stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.