ArticleCurrent genetics1990
The DNA-binding protein RAP1 is required for efficient transcriptional activation of the yeast PYK glycolytic gene.
Article in Current genetics, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 26 citations in OpenAlex.
- Rap1 overexpression boosts triterpenoid saponin production in yeast by enhancing precursor supply and heterologous gene expression.Microbial cell factories · 2025Article
- The CDK8 kinase module: A novel player in the transcription of translation initiation and ribosomal genes.Molecular biology of the cell · 2025Article
- Mediator complex association with constitutively transcribed genes in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Multiple domains of repressor activator protein 1 contribute to facilitated binding of glycolysis regulatory protein 1.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p).Molecular and cellular biology · 1996Article
- Positive and negative transcriptional regulation by the yeast GAL11 protein depends on the structure of the promoter and a combination of cis elements.Molecular & general genetics : MGG · 1994Article
- Compilation of sequence-specific DNA-binding proteins implicated in transcriptional control in fungi.Nucleic acids research · 1993Article
- Concerted action of the transcriptional activators REB1, RAP1, and GCR1 in the high-level expression of the glycolytic gene TPI.Molecular and cellular biology · 1993Article
- Isolation and characterization of the Aspergillus niger pyruvate kinase gene.Current genetics · 1992Article
- Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiae.Molecular and cellular biology · 1992Article
- Yeast glycolytic mRNAs are differentially regulated.Molecular and cellular biology · 1991Article
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4 authors at 1 institution in 1 country.
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Abstract
We show by deletion mutagenesis, followed by in vivo and in vitro analysis, that the binding of a protein factor to the upstream activation sequence (UAS) of the Saccharomyces cerevisiae glycolytic gene PYK, encoding pyruvate kinase, is required for efficient transcription of the corresponding coding region. In addition, gel electrophoretic mobility shift and DNase I protection studies, involving yeast gene products expressed in E. coli, suggest that this trans-acting DNA-binding protein is encoding by the RAP1 gene. The identification of RAP1 binding sites located within the UAS element of the yeast PYK, PGK (phosphoglycerate kinase) and ENO1 (enolase) genes, and in the 5'-upstream region of the ADHI (alcohol dehydrogenase) gene, suggests that a mechanism of coordinate gene expression involving several of the glycolytic genes may exist in yeast.
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