ArticleJournal of immunological methods2011
Optimizing high dimensional gene expression studies for immune response following smallpox vaccination using Taqman® low density immune arrays.
Article in Journal of immunological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Impaired innate, humoral, and cellular immunity despite a take in smallpox vaccine recipients.Vaccine · 2016Article
- An interaction quantitative trait loci tool implicates epistatic functional variants in an apoptosis pathway in smallpox vaccine eQTL data.Genes and immunity · 2016Article
- Molecular network, pathway, and functional analysis of time-dependent gene changes associated with pancreatic cancer susceptibility to oncolytic vaccinia virotherapy.Molecular therapy oncolytics · 2016Article
- Genome-wide characterization of transcriptional patterns in high and low antibody responders to rubella vaccination.PloS one · 2013Article
- High-dimensional gene expression profiling studies in high and low responders to primary smallpox vaccination.The Journal of infectious diseases · 2012Article
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Authors and funding
6 authors.
Funding
Abstract
introductionWe sought to determine the time and vaccinia virus dose combination that would maximize the number of acute immune response changes in response to vaccinia stimulation in preparation for a large gene expression microarray experiment.
methodsPBMCs from ten subjects were exposed to five vaccinia virus doses for three lengths of time. Gene expression was measured for 90 immune response genes via Taqman® Low Density Immune Arrays. Expression data were normalized via model-based non-linear normalization. Linear mixed effects model results were used to standardize changes across genes and determine the time/multiplicity of infection (MOI) combination with the largest number of changes.
resultsThe greatest number of changes occurred with a MOI of 5.0 and exposure time of 48 h. Further inspection revealed that most changes had occurred earlier and faded at this combination. The second highest number of changes was found at a MOI of 0.5 PFU/cell and time of 18 h.
conclusionsWe conclude a time of 18 h with a MOI of 0.5 PFU/cell is the optimal time/MOI combination for the full scale gene expression study. The strategy described herein is a general and resource efficient way to make critical decisions regarding experimental parameters for studies utilizing expensive assays that interrogate a large number of variables.
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