ArticleBMC genomics2011
RNA-seq analyses of blood-induced changes in gene expression in the mosquito vector species, Aedes aegypti.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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Who cites it
90 citing papers in PubMed.
- The clock gene cycle synchronizes circadian metabolic oscillations uniquely in reproductively active Culex pipiens females.npj metabolic health and disease · 2026Article
- Altered histone modifications in Aedes aegypti midguts following Rift Valley fever virus exposure.Scientific reports · 2026Article
- Article
- Temporal changes in amino acid profiles coordinate mosquito physiology and egg maturation in Aedes aegypti.BMC biology · 2025Article
- The effects of mating and blood feeding on the immune defense of female Aedes aegypti mosquitoes.PLoS neglected tropical diseases · 2025Article
- A single-nucleus transcriptomic atlas of the adultbioRxiv : the preprint server for biology · 2025Article
- Altered histone modifications inbioRxiv : the preprint server for biology · 2025Article
- Engineered antibody-mediated broad-spectrum suppression of human arboviruses in the Aedes aegypti vector.Communications biology · 2025Article
- The expanded immunoregulatory protease network in mosquitoes is governed by gene coexpression.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Culex quinquefasciatus Mosquitoes Exposed to a Juvenile Hormone Analog may Take a Bloodmeal While Gravid.Archives of insect biochemistry and physiology · 2025Article
- Comparison of the effect of bacterial stimulation on the global epigenetic landscape and transcription of immune genes in primarily zoophilic members of the Anopheles gambiae complex (Diptera: Culicidae).Molecular and biochemical parasitology · 2024Article
- Article
- Warmer environmental temperature accelerates aging in mosquitoes, decreasing longevity and worsening infection outcomes.Immunity & ageing : I & A · 2024Article
- Niemann-Pick Type C2 Proteins inInternational journal of molecular sciences · 2024Article
- The midgut epithelium of mosquitoes adjusts cell proliferation and endoreplication to respond to physiological challenges.BMC biology · 2024Article
- Introducing an environmental microbiome to axenicApplied and environmental microbiology · 2023Article
- An 11-point time course midgut transcriptome across 72 h after bloodfeeding provides detailed temporal resolution of transcript expression in the arbovirus vector,Genome research · 2023Article
- Sugar restriction and blood ingestion shape divergent immune defense trajectories in the mosquito Aedes aegypti.Scientific reports · 2023Article
- RNA-Seq-Pop: Exploiting the sequence in RNA sequencing-A Snakemake workflow reveals patterns of insecticide resistance in the malaria vector Anopheles gambiae.Molecular ecology resources · 2023Article
- Strong Positive Selection in Aedes aegypti and the Rapid Evolution of Insecticide Resistance.Molecular biology and evolution · 2023Article
30 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
backgroundHematophagy is a common trait of insect vectors of disease. Extensive genome-wide transcriptional changes occur in mosquitoes after blood meals, and these are related to digestive and reproductive processes, among others. Studies of these changes are expected to reveal molecular targets for novel vector control and pathogen transmission-blocking strategies. The mosquito Aedes aegypti (Diptera, Culicidae), a vector of Dengue viruses, Yellow Fever Virus (YFV) and Chikungunya virus (CV), is the subject of this study to look at genome-wide changes in gene expression following a blood meal.
resultsTranscriptional changes that follow a blood meal in Ae. aegypti females were explored using RNA-seq technology. Over 30% of more than 18,000 investigated transcripts accumulate differentially in mosquitoes at five hours after a blood meal when compared to those fed only on sugar. Forty transcripts accumulate only in blood-fed mosquitoes. The list of regulated transcripts correlates with an enhancement of digestive activity and a suppression of environmental stimuli perception and innate immunity. The alignment of more than 65 million high-quality short reads to the Ae. aegypti reference genome permitted the refinement of the current annotation of transcript boundaries, as well as the discovery of novel transcripts, exons and splicing variants. Cis-regulatory elements (CRE) and cis-regulatory modules (CRM) enriched significantly at the 5'end flanking sequences of blood meal-regulated genes were identified.
conclusionsThis study provides the first global view of the changes in transcript accumulation elicited by a blood meal in Ae. aegypti females. This information permitted the identification of classes of potentially co-regulated genes and a description of biochemical and physiological events that occur immediately after blood feeding. The data presented here serve as a basis for novel vector control and pathogen transmission-blocking strategies including those in which the vectors are modified genetically to express anti-pathogen effector molecules.
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