ArticleCell death and differentiation2011
Derivation of neural precursor cells from human ES cells at 3% O(2) is efficient, enhances survival and presents no barrier to regional specification and functional differentiation.
Article in Cell death and differentiation, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Bubble Formation Control: Fabrication of Centimeter-Sized Tissue-Like Constructs by Catalase-Coated Oxygen-Releasing Hydrogel.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Thyroid hormone promotes fetal neurogenesis.JCI insight · 2025Article
- MetaboLINK is a novel algorithm for unveiling cell-specific metabolic pathways in longitudinal datasets.Frontiers in neuroscience · 2024Article
- Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs.International journal of molecular sciences · 2022Article
- Oxygen as a Master Regulator of Human Pluripotent Stem Cell Function and Metabolism.Journal of personalized medicine · 2021Review
- Nup133 and ERα mediate the differential effects of hyperoxia-induced damage in male and female OPCs.Molecular and cellular pediatrics · 2020Article
- Thyroid-related hormones as potential markers of hypoxia/ischemia.Human cell · 2020Article
- Cellular regeneration strategies for macular degeneration: past, present and future.Eye (London, England) · 2018Review
- A PP2A-mediated feedback mechanism controls CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Physiologically normal 5% O2 supports neuronal differentiation and resistance to inflammatory injury in neural stem cell cultures.Journal of neuroscience research · 2015Article
- Low oxygen alters mitochondrial function and response to oxidative stress in human neural progenitor cells.PeerJ · 2015Article
- Targeting the vasculature to improve neural progenitor transplant survival.Translational neuroscience · 2015Review
- Survival of neural progenitors allografted into the CNS of immunocompetent recipients is highly dependent on transplantation site.Cell transplantation · 2014Article
- Influence of oxygen tension on dopaminergic differentiation of human fetal stem cells of midbrain and forebrain origin.PloS one · 2014Article
- Article
- Using human induced pluripotent stem cells to treat retinal disease.Progress in retinal and eye research · 2013Review
- Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone.Journal of proteome research · 2013Article
- Neural precursor cells cultured at physiologically relevant oxygen tensions have a survival advantage following transplantation.Stem cells translational medicine · 2013Article
- Our NIH years: a confluence of beginnings.The Journal of biological chemistry · 2013Article
- High yields of oligodendrocyte lineage cells from human embryonic stem cells at physiological oxygen tensions for evaluation of translational biology.Stem cell reports · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
In vitro stem cell systems traditionally employ oxygen levels that are far removed from the in vivo situation. This study investigates whether an ambient environment containing a physiological oxygen level of 3% (normoxia) enables the generation of neural precursor cells (NPCs) from human embryonic stem cells (hESCs) and whether the resultant NPCs can undergo regional specification and functional maturation. We report robust and efficient neural conversion at 3% O(2), demonstration of tri-lineage potential of resultant NPCs and the subsequent electrophysiological maturation of neurons. We also show that NPCs derived under 3% O(2) can be differentiated long term in the absence of neurotrophins and can be readily specified into both spinal motor neurons and midbrain dopaminergic neurons. Finally, modelling the oxygen stress that occurs during transplantation, we demonstrate that in vitro transfer of NPCs from a 20 to 3% O(2) environment results in significant cell death, while maintenance in 3% O(2) is protective. Together these findings support 3% O(2) as a physiologically relevant system to study stem cell-derived neuronal differentiation and function as well as to model neuronal injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.