ArticleMolecular endocrinology (Baltimore, Md.)2011
Testosterone, not 5α-dihydrotestosterone, stimulates LRH-1 leading to FSH-independent expression of Cyp19 and P450scc in granulosa cells.
Article in Molecular endocrinology (Baltimore, Md.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 63 citations in OpenAlex.
- Hydrogen sulfide donor sodium hydrosulfide modulates ovarian steroidogenesis and follicular integrity in a DHEA-induced rat model of polycystic ovary syndrome.Frontiers in endocrinology · 2026Article
- Insulin-like growth factor 1 enhances follicle-stimulating hormone-induced phosphorylation of GATA4 in rat granulosa cells.Molecular and cellular endocrinology · 2023Article
- Letrozole increases preantral follicle growth and decreases estradiol production without impairing follicle survival.Journal of ovarian research · 2022Article
- Mechanism of negative modulation of FSH signaling by salt-inducible kinases in rat granulosa cells.Frontiers in endocrinology · 2022Article
- Serum testosterone levels are positively associated with serum anti-mullerian hormone levels in infertile women.Scientific reports · 2021Article
- Prenatal low-dose methyltestosterone, but not dihydrotestosterone, treatment induces penile formation in female mice and guinea pigs†.Biology of reproduction · 2020Article
- Androgens Modulate Rat Granulosa Cell Steroidogenesis.Reproductive sciences (Thousand Oaks, Calif.) · 2020Article
- Sp1 regulates steroidogenic genes and LHCGR expression in primary human luteinized granulosa cells.The Journal of steroid biochemistry and molecular biology · 2019Article
- Physiological and Pathological Androgen Actions in the Ovary.Endocrinology · 2019Review
- Antiandrogen Treatment Ameliorates Reproductive and Metabolic Phenotypes in the Letrozole-Induced Mouse Model of PCOS.Endocrinology · 2018Article
- Androgens Regulate Ovarian Gene Expression Through Modulation of Ezh2 Expression and Activity.Endocrinology · 2017Article
- Zinc finger gene 217 (ZNF217) Promoted Ovarian Hyperstimulation Syndrome (OHSS) through Regulating EScientific reports · 2017Article
- Expression pattern of circadian genes and steroidogenesis-related genes after testosterone stimulation in the human ovary.Journal of ovarian research · 2016Article
- Androgens Attenuate Vitamin D Production Induced by UVB Irradiation of the Skin of Male Mice by an Enzymatic Mechanism.The Journal of investigative dermatology · 2015Article
- A Novel Letrozole Model Recapitulates Both the Reproductive and Metabolic Phenotypes of Polycystic Ovary Syndrome in Female Mice.Biology of reproduction · 2015Article
- Genetics of androgen metabolism in women with infertility and hypoandrogenism.Nature reviews. Endocrinology · 2015Review
- Abnormal mitochondrial function and impaired granulosa cell differentiation in androgen receptor knockout mice.International journal of molecular sciences · 2015Article
- 1,25-Dihydroxyvitamin D3 increases testosterone-induced 17beta-estradiol secretion and reverses testosterone-reduced connexin 43 in rat granulosa cells.Reproductive biology and endocrinology : RB&E · 2014Article
- Evidence that increased ovarian aromatase activity and expression account for higher estradiol levels in African American compared with Caucasian women.The Journal of clinical endocrinology and metabolism · 2014Article
- Androgens regulate ovarian follicular development by increasing follicle stimulating hormone receptor and microRNA-125b expression.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Androgens are crucial for normal folliculogenesis and female fertility as evidenced in androgen receptor-null and granulosa cell conditional knockout mice. It is thought, however, that the multiple effects of androgens in the ovary are mainly complementary to the actions of gonadotropins. Using primary rat granulosa cells, we demonstrated that in the absence of gonadotropins, testosterone (T) increases aromatase (Cyp19) and P450 side-change cleavage expression, two enzymes crucial for normal ovarian function. T can be converted into estradiol, a classical estrogen, by Cyp19 and into 5α-dihydrotestosterone, a pure androgen, by 5α-reductase. However, inhibition of Cyp19 and/or 5α-reductase did not prevent the stimulatory effects of T. In contrast, the effect of this steroid was potentiated by blocking 5α-reductase. Additionally, T, not 5α-dihydrotestosterone, stimulates liver receptor homolog-1 (LRH-1) expression, whereas the expression of steroidogenic factor-1 (SF-1) was not affected by either steroid. LRH-1 and SF-1 are transcription factors known to be involved in the regulation of Cyp19. Accordingly, small interference RNA against LRH-1 prevented Cyp19 and P450 side-change cleavage up-regulation whereas anti-SF-1 small interference RNA had no effects. Chromatin immunoprecipitation demonstrated that T stimulation of LRH-1 leads to the recruitment of LRH-1 to the native Cyp19 promoter, which was not affected by cotreatment with 5α-reductase and Cyp19 inhibitors. Finally, gel shift and supershift analysis demonstrated that the androgen receptor binds to an androgen response element located within the LRH-1 promoter. These results provide novel evidence that T has a direct effect on the expression of genes involved in granulosa cell differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.