Trial reportNutrition journal2011
Cruciferous vegetable supplementation in a controlled diet study alters the serum peptidome in a GSTM1-genotype dependent manner.
Trial report in Nutrition journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Proteomic profiling in personalized nutrition: a systematic review and methodological frameworks of randomized controlled trials.Frontiers in nutrition · 2026Pooled it
- Diet rich in high glucoraphanin broccoli reduces plasma LDL cholesterol: Evidence from randomised controlled trials.Molecular nutrition & food research · 2015Trial
- An open label pilot study to evaluate the efficacy of Spanish black radish on the induction of phase I and phase II enzymes in healthy male subjects.BMC complementary and alternative medicine · 2014Trial
- Assessment of DNA damage and repair in adults consuming allyl isothiocyanate or Brassica vegetables.The Journal of nutritional biochemistry · 2013Trial
- Composition of the Gut Microbiome Influences Production of Sulforaphane-Nitrile and Iberin-Nitrile from Glucosinolates in Broccoli Sprouts.Nutrients · 2021Article
- Metabolic Fate of Dietary Glucosinolates and Their Metabolites: A Role for the Microbiome.Frontiers in nutrition · 2021Review
- Targeted proteomic response to coffee consumption.European journal of nutrition · 2020Article
- A Review of Dietary (Phyto)Nutrients for Glutathione Support.Nutrients · 2019Review
- Review
- Integration of Genome-Wide SNP Data and Gene-Expression Profiles Reveals Six Novel Loci and Regulatory Mechanisms for Amino Acids and Acylcarnitines in Whole Blood.PLoS genetics · 2015Article
- The effect of glutathione S-transferase M1 and T1 polymorphisms on blood pressure, blood glucose, and lipid profiles following the supplementation of kale (Brassica oleracea acephala) juice in South Korean subclinical hypertensive patients.Nutrition research and practice · 2015Article
- Modulation of Metabolic Detoxification Pathways Using Foods and Food-Derived Components: A Scientific Review with Clinical Application.Journal of nutrition and metabolism · 2015Review
- Dermcidin expression is associated with disease progression and survival among breast cancer patients.Breast cancer research and treatment · 2014Article
- Inter-individual differences in response to dietary intervention: integrating omics platforms towards personalised dietary recommendations.The Proceedings of the Nutrition Society · 2013Article
- Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse model.Journal of the National Cancer Institute · 2012Article
- Cellular defense system gene expression profiling of human whole blood: opportunities to predict health benefits in response to diet.Advances in nutrition (Bethesda, Md.) · 2012Review
- Proteomic analysis of covalent modifications of tubulins by isothiocyanates.The Journal of nutrition · 2012Article
- Opportunities and challenges for nutritional proteomics in cancer prevention.The Journal of nutrition · 2012Article
- Mechanisms of action of isothiocyanates in cancer chemoprevention: an update.Food & function · 2011Review
- Plant science and human nutrition: challenges in assessing health-promoting properties of phytochemicals.The Plant cell · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundCruciferous vegetable intake is inversely associated with the risk of several cancers. Isothiocyanates (ITC) are hypothesized to be the major bioactive constituents contributing to these cancer-preventive effects. The polymorphic glutathione-S-transferase (GST) gene family encodes several enzymes which catalyze ITC degradation in vivo.
methodsWe utilized high throughput proteomics methods to examine how human serum peptides (the "peptidome") change in response to cruciferous vegetable feeding in individuals of different GSTM1 genotypes. In two randomized, crossover, controlled feeding studies (EAT and 2EAT) participants consumed a fruit- and vegetable-free basal diet and the basal diet supplemented with cruciferous vegetables. Serum samples collected at the end of the feeding period were fractionated and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry spectra were obtained. Peak identification/alignment computer algorithms and mixed effects models were used to analyze the data.
resultsAfter analysis of spectra from EAT participants, 24 distinct peaks showed statistically significant differences associated with cruciferous vegetable intake. Twenty of these peaks were driven by their GSTM1 genotype (i.e., GSTM1+ or GSTM1- null). When data from EAT and 2EAT participants were compared by joint processing of spectra to align a common set, 6 peaks showed consistent changes in both studies in a genotype-dependent manner. The peaks at 6700 m/z and 9565 m/z were identified as an isoform of transthyretin (TTR) and a fragment of zinc α2-glycoprotein (ZAG), respectively.
conclusionsCruciferous vegetable intake in GSTM1+ individuals led to changes in circulating levels of several peptides/proteins, including TTR and a fragment of ZAG. TTR is a known marker of nutritional status and ZAG is an adipokine that plays a role in lipid mobilization. The results of this study present evidence that the GSTM1-genotype modulates the physiological response to cruciferous vegetable intake.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.