ArticleTraffic (Copenhagen, Denmark)2011
Regulation of fusion pore closure and compound exocytosis in neuroendocrine PC12 cells by SCAMP1.
Article in Traffic (Copenhagen, Denmark), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Genomic characteristics of adipose-derived stromal cells induced into neurons based on single-cell RNA sequencing.Heliyon · 2024Article
- Depalmitoylation and cell physiology: APT1 as a mediator of metabolic signals.American journal of physiology. Cell physiology · 2024Review
- Palmitoylation couples insulin hypersecretion with β cell failure in diabetes.Cell metabolism · 2023Article
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- Article
- Proteomic alterations underlie an association with teratozoospermia in obese mice sperm.Reproductive biology and endocrinology : RB&E · 2019Article
- Anaphylactic Degranulation of Mast Cells: Focus on Compound Exocytosis.Journal of immunology research · 2019Review
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- SCAMP5 plays a critical role in synaptic vesicle endocytosis during high neuronal activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Types of DNA methylation status of the interspersed repetitive sequences for LINE-1, Alu, HERV-E and HERV-K in the neutrophils from systemic lupus erythematosus patients and healthy controls.Journal of human genetics · 2014Article
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- FIP3-endosome-dependent formation of the secondary ingression mediates ESCRT-III recruitment during cytokinesis.Nature cell biology · 2012Article
- Regulated exocytosis: novel insights from intravital microscopy.Traffic (Copenhagen, Denmark) · 2012Review
- Linking differences in membrane tension with the requirement for a contractile actomyosin scaffold during exocytosis in salivary glands.Communicative & integrative biology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
During exocytosis, neuroendocrine cells can achieve partial release of stored secretory products from dense core vesicles (DCVs) by coupling endocytosis directly at fusion sites and without full discharge. The physiological role of partial secretion is of substantial interest. Much is known about SNARE-mediated initiation of exocytosis and dynamin-mediated completion of endocytosis, but little is known about coupling events. We have used real-time microscopy to examine the role of secretory carrier membrane protein SCAMP1 in exo-endocytic coupling in PC12 cells. While reduced SCAMP1 expression is known to impede dilation of newly opened fusion pores during onset of DCV exocytosis, we now show that SCAMP1 deficiency also inhibits closure of fusion pores after they have opened. Inhibition causes accumulation of fusion figures at the plasma membrane. Closure is recovered by restoring expression and accelerated slightly by overexpression. Interestingly, inhibited pore closure resulting from loss of SCAMP1 appears to increase secondary fusion of DCVs to already-fused DCVs (compound exocytosis). Unexpectedly, reinternalization of expanded DCV membranes following compound exocytosis appears to proceed normally in SCAMP1-deficient cells. SCAMP1's apparent dual role in facilitating dilation and closure of fusion pores implicates its function in exo-endocytic coupling and in the regulation of partial secretion. Secondarily, SCAMP1 may serve to limit the extent of compound exocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.