ArticleGenetics2011
Augmented annotation of the Schizosaccharomyces pombe genome reveals additional genes required for growth and viability.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Review
- Measuring the impact of gene prediction on gene loss estimates in Eukaryotes by quantifying falsely inferred absences.PLoS computational biology · 2019Article
- A genome-wide screen to identify genes controlling the rate of entry into mitosis in fission yeast.Cell cycle (Georgetown, Tex.) · 2016Article
- Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.Yeast (Chichester, England) · 2016Article
- AnABlast: a new in silico strategy for the genome-wide search of novel genes and fossil regions.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
- Cwf16p Associating with the Nineteen Complex Ensures Ordered Exon Joining in Constitutive Pre-mRNA Splicing in Fission Yeast.PloS one · 2015Article
- Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.G3 (Bethesda, Md.) · 2014Article
- Developmental transcriptome analysis of human erythropoiesis.Human molecular genetics · 2014Article
- On the extent and role of the small proteome in the parasitic eukaryote Trypanosoma brucei.BMC biology · 2014Article
- The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life.Natural product reports · 2014Review
- Deducing protein function by forensic integrative cell biology.PLoS biology · 2013Article
- Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3(Alp6).Molecular biology of the cell · 2013Article
- A novel factor Iss10 regulates Mmi1-mediated selective elimination of meiotic transcripts.Nucleic acids research · 2013Article
- Deep coverage of the Escherichia coli proteome enables the assessment of false discovery rates in simple proteogenomic experiments.Molecular & cellular proteomics : MCP · 2013Article
- Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly.Molecular biology of the cell · 2013Article
- Lariat sequencing in a unicellular yeast identifies regulated alternative splicing of exons that are evolutionarily conserved with humans.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Extensive mass spectrometry-based analysis of the fission yeast proteome: the Schizosaccharomyces pombe PeptideAtlas.Molecular & cellular proteomics : MCP · 2013Article
- Linkers of cell polarity and cell cycle regulation in the fission yeast protein interaction network.PLoS computational biology · 2012Article
- Article
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Authors and funding
8 authors.
Funding
Abstract
Genome annotation is a synthesis of computational prediction and experimental evidence. Small genes are notoriously difficult to detect because the patterns used to identify them are often indistinguishable from chance occurrences, leading to an arbitrary cutoff threshold for the length of a protein-coding gene identified solely by in silico analysis. We report a systematic reappraisal of the Schizosaccharomyces pombe genome that ignores thresholds. A complete six-frame translation was compared to a proteome data set, the Pfam domain database, and the genomes of six other fungi. Thirty-nine novel loci were identified. RT-PCR and RNA-Seq confirmed transcription at 38 loci; 33 novel gene structures were delineated by 5' and 3' RACE. Expression levels of 14 transcripts fluctuated during meiosis. Translational evidence for 10 genes, evolutionary conservation data supporting 35 predictions, and distinct phenotypes upon ORF deletion (one essential, four slow-growth, two delayed-division phenotypes) suggest that all 39 predictions encode functional proteins. The popularity of S. pombe as a model organism suggests that this augmented annotation will be of interest in diverse areas of molecular and cellular biology, while the generality of the approach suggests widespread applicability to other genomes.
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