ArticleDiabetes2011
Combined small interfering RNA therapy and in vivo magnetic resonance imaging in islet transplantation.
Article in Diabetes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- Omniparticle Contrast Agent for Multimodal Imaging: Synthesis and Characterization in an Animal Model.Molecular imaging and biology · 2023Article
- Synthesis of siRNA-Conjugated Dextran-Coated Iron Oxide Nanoparticles for Islet Protection During Transplantation and Noninvasive Imaging.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering.Nanomaterials (Basel, Switzerland) · 2021Review
- Artificial Intelligence Analysis of Magnetic Particle Imaging for Islet Transplantation in a Mouse Model.Molecular imaging and biology · 2021Article
- 3DFrontiers in cell and developmental biology · 2021Article
- A Strategy to Simultaneously Cure Type 1 Diabetes and Diabetic Nephropathy by Transplant of Composite Islet-Kidney Grafts.Frontiers in endocrinology · 2021Review
- Protection of Pancreatic Islets Using Theranostic Silencing Nanoparticles in a Baboon Model of Islet Transplantation.Diabetes · 2020Article
- Current Progress and Perspective: Clinical Imaging of Islet Transplantation.Life (Basel, Switzerland) · 2020Review
- miR-216a-targeting theranostic nanoparticles promote proliferation of insulin-secreting cells in type 1 diabetes animal model.Scientific reports · 2020Article
- Islet Transplantation Imaging in vivo.Diabetes, metabolic syndrome and obesity : targets and therapy · 2020Review
- Magnetic particle imaging of islet transplantation in the liver and under the kidney capsule in mouse models.Quantitative imaging in medicine and surgery · 2018Article
- Novel targeted therapy for neuroblastoma: silencing the MXD3 gene using siRNA.Pediatric research · 2017Article
- Recent progress in the use and tracking of transplanted islets as a personalized treatment for type 1 diabetes.Expert review of precision medicine and drug development · 2017Article
- Transgene and islet cell delivery systems using nano-sized carriers for the treatment of diabetes mellitus.Nano reviews & experiments · 2017Review
- Diagnostic and prognostic utility of non-invasive imaging in diabetes management.World journal of diabetes · 2015Review
- Combination treatment with theranostic nanoparticles for glioblastoma sensitization to TMZ.Molecular imaging and biology · 2014Article
- Article
- Recent advances in superparamagnetic iron oxide nanoparticles for cellular imaging and targeted therapy research.Current pharmaceutical design · 2013Review
- Multimodality imaging of RNA interference.Current medicinal chemistry · 2013Review
- A theranostic small interfering RNA nanoprobe protects pancreatic islet grafts from adoptively transferred immune rejection.Diabetes · 2012Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveRecent advances in human islet transplantation are hampered by significant graft loss shortly after transplantation and inability to follow islet fate directly. Both issues were addressed by utilizing a dual-purpose therapy/imaging small interfering RNA (siRNA)-nanoparticle probe targeting apoptotic-related gene caspase-3. We expect that treatment with the probe would result in significantly better survival of transplanted islets, which could be monitored by in vivo magnetic resonance imaging (MRI). RESEARCH DESIGN AND
methodsWe synthesized a probe consisting of therapeutic (siRNA to human caspase-3) and imaging (magnetic iron oxide nanoparticles, MN) moieties. In vitro testing of the probe included serum starvation of the islets followed by treatment with the probe. Caspase-3 gene silencing and protein expression were determined by RT-PCR and Western blot, respectively. In vivo studies included serial MRI of NOD-SCID mice transplanted with MN-small interfering (si)Caspase-3-labeled human islets under the left kidney capsule and MN-treated islets under the right kidney capsule.
resultsTreatment with MN-siCaspase-3 probe resulted in decrease of mRNA and protein expression in serum-starved islets compared with controls. In vivo MRI showed that there were significant differences in the relative volume change between MN-siCaspase-3-treated grafts and MN-labeled grafts. Histology revealed decreased caspase-3 expression and cell apoptosis in MN-siCaspase-3-treated grafts compared with the control side.
conclusionsOur data show the feasibility of combining siRNA therapy and in vivo monitoring of transplanted islets in mice. We observed a protective effect of MN-siCaspase-3 in treated islets both in vitro and in vivo. This study could potentially aid in increasing the success of clinical islet transplantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.