ArticleJournal of clinical microbiology2011
Isothermal microcalorimetry to study drugs against Schistosoma mansoni.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 61 citations in OpenAlex.
- A phenotypic screen of the Global Health Priority Box identifies an insecticide with anthelmintic activity.Parasites & vectors · 2024Article
- A self-purifying microfluidic system for identifying drugs acting against adult schistosomes.Royal Society open science · 2022Article
- Docking-Based Virtual Screening Enables Prioritizing Protein Kinase Inhibitors WithFrontiers in cellular and infection microbiology · 2022Article
- Identification of hit compounds with anti-schistosomal activity on in vitro generated juvenile worms in cell-free medium.PLoS neglected tropical diseases · 2021Article
- Host-Specific Serum Factors Control the Development and Survival ofFrontiers in immunology · 2021Article
- Schistosomiasis Drug Discovery in the Era of Automation and Artificial Intelligence.Frontiers in immunology · 2021Review
- Parallelized Impedance-Based Platform for Continuous Dose-Response Characterization of Antischistosomal Drugs.Advanced biosystems · 2020Article
- Antifungal Susceptibility Testing: Current Approaches.Clinical microbiology reviews · 2020Review
- Life cycle maintenance and drug-sensitivity assays for early drug discovery in Schistosoma mansoni.Nature protocols · 2019Article
- A novel cell-free method to culture Schistosoma mansoni from cercariae to juvenile worm stages for in vitro drug testing.PLoS neglected tropical diseases · 2019Article
- Article
- Assessment of tegumental damage to Schistosoma mansoni and S. haematobium after in vitro exposure to ferrocenyl, ruthenocenyl and benzyl derivatives of oxamniquine using scanning electron microscopy.Parasites & vectors · 2018Article
- Isothermal microcalorimetry - A quantitative method to monitor Trypanosoma congolense growth and growth inhibition by trypanocidal drugs in real time.International journal for parasitology. Drugs and drug resistance · 2018Article
- Susceptibility Testing of Medically Important Parasites.Clinical microbiology reviews · 2017Review
- Development of a movement-based in vitro screening assay for the identification of new anti-cestodal compounds.PLoS neglected tropical diseases · 2017Article
- Evaluation of the Antibacterial Effects of Flavonoid Combination from the Leaves ofFrontiers in pharmacology · 2017Article
- Modern approaches to accelerate discovery of new antischistosomal drugs.Expert opinion on drug discovery · 2016Review
- Fluorescence/luminescence-based markers for the assessment of Schistosoma mansoni schistosomula drug assays.Parasites & vectors · 2015Article
- Lactate as a novel quantitative measure of viability in Schistosoma mansoni drug sensitivity assays.Antimicrobial agents and chemotherapy · 2015Article
- Development and validation of a luminescence-based, medium-throughput assay for drug screening in Schistosoma mansoni.PLoS neglected tropical diseases · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative antischistosomal drugs are required since praziquantel is virtually the only drug available for treatment and morbidity control of schistosomiasis. Manual microscopic reading is the current "gold standard" to assess the in vitro antischistosomal properties of test drugs; however, it is labor-intensive, subjective, and difficult to standardize. Hence, there is a need to develop novel tools for antischistosomal drug discovery. The in vitro effects of praziquantel, oxamniquine, artesunate, and mefloquine on metabolic activity and parasite motility of Schistosoma mansoni (newly transformed schistosomula [NTS] and 49-day-old adult worms) were studied using isothermal microcalorimetry (IMC). Results were compared to morphological readouts of viability. Results obtained for the four drugs tested with phenotypic evaluation by microscopy and IMC showed a good correlation, but IMC also identified drug effects that were not visible by microscopic evaluation, and IMC precisely determined the onset of action of the test drugs. Similar sensitivities on NTS and adult schistosomes were observed for praziquantel and mefloquine, while slight differences in the drug susceptibilities of the two developmental stages were noted with oxamniquine and artesunate. IMC is a useful tool for antischistosomal drug discovery that should be further validated. In addition, our data support the use of NTS in in vitro antischistosomal drug assays.
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