ArticleJournal of lipid research2011
Determination of lipoprotein lipase activity using a novel fluorescent lipase assay.
Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Angiopoietin-like 3 monomers are abundant in human plasma but are unable to inhibit endothelial lipase.JCI insight · 2025Article
- Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets.Nature communications · 2025Article
- S-acylation of ATGL is required for lipid droplet homoeostasis in hepatocytes.Nature metabolism · 2024Article
- A miniaturised semi-dynamic in-vitro model of human digestion.Scientific reports · 2024Article
- Identification of a Gain-of-FunctionCirculation · 2022Article
- A Critical Evaluation of Terpenoid Signaling at Cannabinoid CB1 Receptors in a Neuronal Model.Molecules (Basel, Switzerland) · 2022Article
- Using Synthetic ApoC-II Peptides and nAngptl4 Fragments to Measure Lipoprotein Lipase Activity in Radiometric and Fluorescent Assays.Frontiers in cardiovascular medicine · 2022Article
- CREBH normalizes dyslipidemia and halts atherosclerosis in diabetes by decreasing circulating remnant lipoproteins.The Journal of clinical investigation · 2021Article
- Regulation of plasma triglyceride partitioning by adipose-derived ANGPTL4 in mice.Scientific reports · 2021Article
- High-Throughput, Fluorescence-Based Esterase Activity Assay for Assessing Polysorbate Degradation Risk during Biopharmaceutical Development.Pharmaceutical research · 2021Article
- Angiopoietin-like protein 8 differentially regulates ANGPTL3 and ANGPTL4 during postprandial partitioning of fatty acids.Journal of lipid research · 2020Article
- A novel NanoBiT-based assay monitors the interaction between lipoprotein lipase and GPIHBP1 in real time.Journal of lipid research · 2020Article
- Endocytosis of very low-density lipoproteins: an unexpected mechanism for lipid acquisition by breast cancer cells.Journal of lipid research · 2020Article
- Simple and rapid real-time monitoring of LPL activityMethodsX · 2020Article
- Fluorescent substrates for haloalkane dehalogenases: Novel probes for mechanistic studies and protein labeling.Computational and structural biotechnology journal · 2020Article
- Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition.Scientific reports · 2019Article
- No interactions between heparin and atacicept, an antagonist of B cell survival cytokines.British journal of pharmacology · 2019Article
- The Effect of Acute Continuous Hypoxia on Triglyceride Levels in Constantly Fed Healthy Men.Frontiers in physiology · 2019Article
- Cell therapy could be a potential way to improve lipoprotein lipase deficiency.Lipids in health and disease · 2017Article
- ANGPTL8 promotes the ability of ANGPTL3 to bind and inhibit lipoprotein lipase.Molecular metabolism · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
A novel, real-time, homogeneous fluorogenic lipoprotein lipase (LPL) assay was developed using a commercially available substrate, the EnzChek lipase substrate, which is solubilized in Zwittergent. The triglyceride analog substrate does not fluoresce, owing to apposition of fluorescent and fluorescent quenching groups at the sn-1 and sn-2 positions, respectively, fluorescence becoming unquenched upon release of the sn-1 BODIPY FA derivative following hydrolysis. Increase in fluorescence intensity at 37°C was proportional to LPL concentration. The assay was more sensitive than a similar assay using 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin ester) and was validated in biological samples, including determination of LPL-specific activity in postheparin mouse plasma. The simplicity and reproducibility of the assay make it ideal for in vitro, high-throughput screening for inhibitors and activators of LPL, thus expediting discovery of drugs of potential clinical value.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.