ArticleBMC genomics2011
Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Toward a 4D genome annotation of CHO cells for biomanufacturing.Trends in biotechnology · 2026Review
- Generation and characterization of a HEK293 cell line optimized for recombinant adeno-associated virus production.Biotechnology reports (Amsterdam, Netherlands) · 2026Article
- Next-generation sequencing: A powerful multi-purpose tool in cell line development for biologics production.Computational and structural biotechnology journal · 2025Review
- Glutamine synthetase (GS) knockout (KO) using CRISPR/Cpf1 diversely enhances selection efficiency of CHO cells expressing therapeutic antibodies.Scientific reports · 2023Article
- High Throughput miRNA Screening Identifies miR-574-3p Hyperproductive Effect in CHO Cells.Biomolecules · 2021Article
- Evolution from adherent to suspension: systems biology of HEK293 cell line development.Scientific reports · 2020Article
- Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation.GigaScience · 2018Article
- Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data.BMC systems biology · 2017Article
- Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy.BMC genomics · 2015Article
- Sequencing, annotation and analysis of the Syrian hamster (Mesocricetus auratus) transcriptome.PloS one · 2014Article
- Construction of a public CHO cell line transcript database using versatile bioinformatics analysis pipelines.PloS one · 2014Article
- CHO microRNA engineering is growing up: recent successes and future challenges.Biotechnology advances · 2013Review
- Integrated miRNA, mRNA and protein expression analysis reveals the role of post-transcriptional regulation in controlling CHO cell growth rate.BMC genomics · 2012Article
- Proteomic analysis of Chinese hamster ovary cells.Journal of proteome research · 2012Article
- Profiling conserved microRNA expression in recombinant CHO cell lines using Illumina sequencing.Biotechnology and bioengineering · 2012Article
- Engineering genes for predictable protein expression.Protein expression and purification · 2012Review
- Genomics in mammalian cell culture bioprocessing.Biotechnology advancesReview
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChinese hamster ovary (CHO) cells are among the most widely used hosts for therapeutic protein production. Yet few genomic resources are available to aid in engineering high-producing cell lines.
resultsHigh-throughput Illumina sequencing was used to generate a 1x genomic coverage of an engineered CHO cell line expressing secreted alkaline phosphatase (SEAP). Reference-guided alignment and assembly produced 3.57 million contigs and CHO-specific sequence information for ~ 18,000 mouse and ~ 19,000 rat orthologous genes. The majority of these genes are involved in metabolic processes, cellular signaling, and transport and represent attractive targets for cell line engineering.
conclusionsThis demonstrates the applicability of next-generation sequencing technology and comparative genomic analysis in the development of CHO genomic resources.
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