ArticleAnalytical chemistry2011
Compositional mapping of the surface and interior of mammalian cells at submicrometer resolution.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- On-Tip Polymerization Method for Multimodal Characterization of Nanoparticles with Electron/Ion Imaging and Atom Probe Tomography.Small methods · 2026Article
- FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins in cells of different lineages.Journal of lipid research · 2023Article
- Nanocapillary sampling coupled to liquid chromatography mass spectrometry delivers single cell drug measurement and lipid fingerprints.The Analyst · 2023Article
- Depth correction of 3D NanoSIMS images using secondary electron pixel intensities.Biointerphases · 2021Article
- Effective processing and evaluation of chemical imaging data with respect to morphological features of the zebrafish embryo.Analytical and bioanalytical chemistry · 2021Article
- Single-Cell Mass Spectrometry Reveals Changes in Lipid and Metabolite Expression in RAW 264.7 Cells upon Lipopolysaccharide Stimulation.Journal of the American Society for Mass Spectrometry · 2018Article
- Visualization of metallodrugs in single cells by secondary ion mass spectrometry imaging.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Review
- Near-Atomic Three-Dimensional Mapping for Site-Specific Chemistry of 'Superbugs'.Nano letters · 2016Article
- Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin.Scientific reports · 2016Article
- Focused ion beams in biology.Nature methods · 2015Review
- Mass spectrometry imaging with high resolution in mass and space.Histochemistry and cell biology · 2013Review
- Correlated imaging--a grand challenge in chemical analysis.The Analyst · 2013Review
- Multivariate analysis of ToF-SIMS data from multicomponent systems: the why, when, and how.Biointerphases · 2012Review
- Mass spectrometry imaging and profiling of single cells.Journal of proteomics · 2012Review
- Chemical mapping of mammalian cells by atom probe tomography.Journal of structural biology · 2012Article
- Chemical analysis of single cells.Analytical chemistry · 2011Review
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
We present progress toward imaging of chemical species within intact mammalian cells using secondary ion mass spectrometry, including the simultaneous mapping of subcellular elemental and molecular species along with intrinsic membrane-specific cellular markers. Results from imaging both the cell surface and cell interior exposed by site-specific focused ion beam milling demonstrate that in-plane resolutions of approximately 400-500 nm can be achieved. The results from mapping cell surface phosphatidylcholine and several other molecular ions present in the cells establish that spatially resolved chemical signatures of individual cells can be derived from novel multivariate analysis and classification of the molecular images obtained at different m/z ratios. The methods we present here for specimen preparation and chemical imaging of cell interiors provide the foundation for obtaining 3D molecular maps of unstained mammalian cells, with particular relevance for probing the subcellular distributions of small molecules, such as drugs and metabolites.
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Registered trials
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