ArticleBiochemistry2011
A kinetic aggregation assay allowing selective and sensitive amyloid-β quantification in cells and tissues.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Article
- Mucin-TypeFrontiers in chemistry · 2022Article
- Perspectives on theToxins · 2021Review
- Proteopathic Seed Amplification Assays for Neurodegenerative Disorders.Clinics in laboratory medicine · 2020Review
- Are We Ready for Detecting α-Synuclein Prone to Aggregation in Patients? The Case of "Protein-Misfolding Cyclic Amplification" and "Real-Time Quaking-Induced Conversion" as Diagnostic Tools.Frontiers in neurology · 2018Review
- Insight of brain degenerative protein modifications in the pathology of neurodegeneration and dementia by proteomic profiling.Molecular brain · 2016Review
- Prions: Beyond a Single Protein.Clinical microbiology reviews · 2016Review
- Conversion of Synthetic Aβ to In Vivo Active Seeds and Amyloid Plaque Formation in a Hippocampal Slice Culture Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation.The journal of physical chemistry. B · 2016Article
- Competitive Mirror Image Phage Display Derived Peptide Modulates Amyloid Beta Aggregation and Toxicity.PloS one · 2016Article
- Unfolded protein response-induced ERdj3 secretion links ER stress to extracellular proteostasis.The EMBO journal · 2015Article
- Proteopathic tau seeding predicts tauopathy in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Mechanisms of transthyretin inhibition of β-amyloid aggregation in vitro.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Toward the molecular mechanism(s) by which EGCG treatment remodels mature amyloid fibrils.Journal of the American Chemical Society · 2013Article
- Protein misfolding detected early in pathogenesis of transgenic mouse model of Huntington disease using amyloid seeding assay.The Journal of biological chemistry · 2012Article
- Concentration-dependent transitions govern the subcellular localization of islet amyloid polypeptide.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Chemical and biological approaches for adapting proteostasis to ameliorate protein misfolding and aggregation diseases: progress and prognosis.Cold Spring Harbor perspectives in biology · 2011Review
- Soluble Aβ seeds are potent inducers of cerebral β-amyloid deposition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The process of amyloid-β (Aβ) fibril formation is genetically and pathologically linked to Alzheimer's disease (AD). Thus, a selective and sensitive method for quantifying Aβ fibrils in complex biological samples allows a variety of hypotheses to be tested. Herein, we report the basis for a quantitative in vitro kinetic aggregation assay that detects seeding-competent Aβ aggregates in mammalian cell culture media, in Caenorhabditis elegans lysate, and in mouse brain homogenate. Sonicated, proteinase K-treated Aβ fibril-containing tissue homogenates or cell culture media were added to an initially monomeric Aβ(1-40) reporter peptide to seed an in vitro nucleated aggregation reaction. The reduction in the half-time (t(50)) of the amyloid growth phase is proportional to the quantity of seeding-competent Aβ aggregates present in the biological sample. An ion-exchange resin amyloid isolation strategy from complex biological samples is demonstrated as an alternative for improving the sensitivity and linearity of the kinetic aggregation assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.