ArticleThe protein journal2011
Characterization of a single-stranded DNA binding protein from Salmonella enterica serovar Typhimurium LT2.
Article in The protein journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
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- Binding Pattern and Structural Interactome of the Anticancer Drug 5-Fluorouracil: A Critical Review.International journal of molecular sciences · 2024Review
- The Inhibitory Effects and Cytotoxic Activities of the Stem Extract ofPlants (Basel, Switzerland) · 2022Article
- Crystal Structure of an SSB Protein fromInternational journal of molecular sciences · 2022Article
- Characterization of the Chimeric PriB-SSBc Protein.International journal of molecular sciences · 2021Article
- Characterization of an SSB-dT25 complex: structural insights into the S-shaped ssDNA binding conformation.RSC advances · 2019Article
- The glycine-rich flexible region in SSB is crucial for PriA stimulation.RSC advances · 2018Article
- SAAV2152 is a single-stranded DNA binding protein: the third SSB inOncotarget · 2018Article
- Staphylococcus aureus single-stranded DNA-binding protein SsbA can bind but cannot stimulate PriA helicase.PloS one · 2017Article
- C-terminal domain swapping of SSB changes the size of the ssDNA binding site.BioMed research international · 2014Article
- Yeast two-hybrid analysis of PriB-interacting proteins in replication restart primosome: a proposed PriB-SSB interaction model.The protein journal · 2013Article
- ATP-dependent RecG helicase is required for the transcriptional regulator OxyR function in Pseudomonas species.The Journal of biological chemistry · 2012Article
- Characterization of flavonol inhibition of DnaB helicase: real-time monitoring, structural modeling, and proposed mechanism.Journal of biomedicine & biotechnology · 2012Article
- Characterization of the single stranded DNA binding protein SsbB encoded in the Gonoccocal Genetic Island.PloS one · 2012Article
- Biochemical characterization of allantoinase from Escherichia coli BL21.The protein journal · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-stranded DNA-binding protein (SSB) plays an important role in DNA metabolism, such as DNA replication, repair, and recombination, and is essential for cell survival. We characterized the single-stranded DNA (ssDNA)-binding properties of Salmonella enterica serovar Typhimurium LT2 SSB (StSSB) by using fluorescence quenching measurements and electrophoretic mobility shift analysis (EMSA). Analysis of purified StSSB by gel filtration chromatography showed a stable tetramer in solution. In fluorescence titrations, StSSB bound to 21-38 nucleotides (nt) per tetramer depending on the salt concentration. Using EMSA, we characterized the stoichiometry of StSSB complexed with a series of ssDNA homopolymers, and the size of the binding site was determined to be 22 ± 1 nt. Furthermore, EMSA results indicated that the dissociation constants of StSSB for the first tetramer were less than that for the second tetramer. On the basis of these biophysical analyses, the ssDNA binding-mode of StSSB is expected to be noncooperative.
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