ArticleNature communications2011
An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells.
Article in Nature communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
87 citing papers in PubMed, 172 citations in OpenAlex.
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- Network-informed discovery of multidrug combinations for ERα+/HER2-/PI3Kα-mutant breast cancer.Cellular and molecular life sciences : CMLS · 2023Article
- An optimized cocktail of small molecule inhibitors promotes the maturation of dendritic cells in GM-CSF mouse bone marrow culture.Frontiers in immunology · 2023Article
- Article
- Long-term maintenance of human endometrial epithelial stem cells and their therapeutic effects on intrauterine adhesion.Cell & bioscience · 2022Article
- Small-molecule inhibitor cocktail promotes the proliferation of pre-existing liver progenitor cells.Stem cell reports · 2022Article
- Improving the immunomodulatory function of mesenchymal stem cells by defined chemical approach.Frontiers in immunology · 2022Article
- Harnessing features of adaptive NK cells to generate iPSC-derived NK cells for enhanced immunotherapy.Cell stem cell · 2021Article
- Personalised, Rational, Efficacy-Driven Cancer Drug DosingFrontiers in digital health · 2021Article
- iPSC-derived NK cells maintain high cytotoxicity and enhance in vivo tumor control in concert with T cells and anti-PD-1 therapy.Science translational medicine · 2020Article
- Potential of transposon-mediated cellular reprogramming towards cell-based therapies.World journal of stem cells · 2020Review
- Differential Evolution for the Optimization of DMSO-Free Cryoprotectants: Influence of Control Parameters.Journal of biomechanical engineering · 2020Article
- Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture.Stem cell reports · 2020Article
- Small-molecule-mediated reprogramming: a silver lining for regenerative medicine.Experimental & molecular medicine · 2020Review
- Inducing human induced pluripotent stem cell differentiation through embryoid bodies: A practical and stable approach.World journal of stem cells · 2020Review
- Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells.Journal of visualized experiments : JoVE · 2020Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
A major challenge in stem cell-mediated regenerative medicine is the development of defined culture systems for the maintenance of clinical-grade human embryonic stem (hES) cells. Here, we identify, using a feedback system control scheme, a unique combination of three small molecule inhibitors that enables the maintenance of hES cells on a fibronectin-coated surface through single cell passaging. After 20 passages, the undifferentiated state of the hES cells was confirmed by OCT4, SSEA4 and NANOG expressions, whereas their pluripotent potential and genetic integrity were demonstrated by teratoma formation and normal karyotype, respectively. Our study attests to the power of the feedback system control scheme to quickly pinpoint optimal conditions for desired biological activities, and provides a chemically defined, scalable and single cell passaging culture system for hES cells.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.