ArticleMolecular therapy : the journal of the American Society of Gene Therapy2011
Functionalized scaffold-mediated interleukin 10 gene delivery significantly improves survival rates of stem cells in vivo.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Advanced Immunomodulatory Biomaterials for Therapeutic Applications.Advanced healthcare materials · 2025Review
- Gene Augmentation Techniques to Stimulate Wound Healing Process: Progress and Prospects.Current gene therapy · 2025Review
- Peptide-based immunotherapy in lupus: Where are we now?Rheumatology and immunology research · 2023Review
- Targeting Inflammation and Regeneration: Scaffolds, Extracellular Vesicles, and Nanotechnologies as Cell-Free Dual-Target Therapeutic Strategies.International journal of molecular sciences · 2022Review
- Stem Cell Fate and Immunomodulation Promote Bone RegenerationFrontiers in bioengineering and biotechnology · 2022Article
- The State of the Art and Prospects for Osteoimmunomodulatory Biomaterials.Materials (Basel, Switzerland) · 2021Review
- Emerging Role of IL-10 in Hypertrophic Scars.Frontiers in medicine · 2020Review
- Biomaterials: Foreign Bodies or Tuners for the Immune Response?International journal of molecular sciences · 2019Review
- Anti-Folate Receptor-α IgE but not IgG Recruits Macrophages to Attack Tumors via TNFα/MCP-1 Signaling.Cancer research · 2017Article
- Naturally derived biomaterials for addressing inflammation in tissue regeneration.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Controlled release strategies for modulating immune responses to promote tissue regeneration.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Immune modulation to improve tissue engineering outcomes for cartilage repair in the osteoarthritic joint.Tissue engineering. Part B, Reviews · 2015Review
- Biomaterial-mediated modification of the local inflammatory environment.Frontiers in bioengineering and biotechnology · 2015Review
- Tissue-engineered cartilage with inducible and tunable immunomodulatory properties.Biomaterials · 2014Article
- A collagen-based scaffold delivering exogenous microrna-29B to modulate extracellular matrix remodeling.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- Decrease in IL-10 and increase in TNF-α levels in renal tissues during systemic inhibition of nitric oxide in anesthetized mice.Physiological reports · 2014Article
- Modulation of leukocyte infiltration and phenotype in microporous tissue engineering scaffolds via vector induced IL-10 expression.Biomaterials · 2014Article
- Collagen: finding a solution for the source.Tissue engineering. Part A · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
While stem cell transplantation could potentially treat a variety of disorders, clinical studies have not yet demonstrated conclusive benefits. This may be partly because transplanted stem cells have low survival rates, potentially due to host inflammation. The system described herein used two different gene therapy techniques to improve retention of rat mesenchymal stem cells. In the first, stem cells were transfected with interleukin-10 (IL-10) before being loaded into a collagen scaffold. In the second, unmodified stem cells were loaded into a collagen scaffold along with polymer-complexed IL-10 plasmids. The scaffolds were surgically implanted into the dorsum of syngeneic rats. At each endpoint, the scaffolds were explanted and cell retention, IL-10 level and inflammatory response were quantified. All treatment groups had statistically significant increases in cell retention after 7 days, but the group treated with 2 µg of IL-10 polyplexes had a significant improvement even at 21 days. This cell retention was associated with increased IL-10 and decreased levels of proinflammatory cytokines and apoptosis. The primary effect on the inflammatory response appeared to be on macrophage differentiation, encouraging the regulatory phenotype over the cytotoxic lineage. Improving cell survival may be an important step toward realization of the therapeutic potential of stem cells.
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Registered trials
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