ArticleIslets
A novel fluorescence imaging approach for comparative measurements of pancreatic islet function in vitro.
Article in Islets. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Synchrotron fluorescence imaging of individual mouse beta-cells reveals changes in zinc, calcium, and iron in a model of low-grade inflammation.Metallomics : integrated biometal science · 2021Article
- Postnatal maturation of calcium signaling in islets of Langerhans from neonatal mice.Cell calcium · 2021Article
- A Practical Guide to Rodent Islet Isolation and Assessment Revisited.Biological procedures online · 2021Review
- Chronic stimulation induces adaptive potassium channel activity that restores calcium oscillations in pancreatic islets in vitro.American journal of physiology. Endocrinology and metabolism · 2020Article
- Intact pancreatic islets and dispersed beta-cells both generate intracellular calcium oscillations but differ in their responsiveness to glucose.Cell calcium · 2019Article
- Reducing Glucokinase Activity Restores Endogenous Pulsatility and Enhances Insulin Secretion in Islets From db/db Mice.Endocrinology · 2018Article
- Local tissue manipulation via a force- and pressure-controlled AFM micropipette for analysis of cellular processes.Scientific reports · 2018Article
- Metformin Inhibits Mouse Islet Insulin Secretion and Alters Intracellular Calcium in a Concentration-Dependent and Duration-Dependent Manner near the Circulating Range.Journal of diabetes research · 2018Article
- Islet Hypersensitivity to Glucose Is Associated With Disrupted Oscillations and Increased Impact of Proinflammatory Cytokines in Islets From Diabetes-Prone Male Mice.Endocrinology · 2016Article
- Considerations for Defining Cytokine Dose, Duration, and Milieu That Are Appropriate for Modeling Chronic Low-Grade Inflammation in Type 2 Diabetes.Journal of diabetes research · 2016Review
- An Islet-Targeted Genome-Wide Association Scan Identifies Novel Genes Implicated in Cytokine-Mediated Islet Stress in Type 2 Diabetes.Endocrinology · 2015Article
- Stress-induced dissociations between intracellular calcium signaling and insulin secretion in pancreatic islets.Cell calcium · 2015Article
- Increased serum CXCL1 and CXCL5 are linked to obesity, hyperglycemia, and impaired islet function.The Journal of endocrinology · 2014Article
- Circulating levels of IL-1B+IL-6 cause ER stress and dysfunction in islets from prediabetic male mice.Endocrinology · 2013Article
- Salivary gland hypofunction induced by activation of innate immunity is dependent on type I interferon signaling.Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology · 2013Article
- The central role of calcium in the effects of cytokines on beta-cell function: implications for type 1 and type 2 diabetes.Cell calcium · 2011Review
- Accurate measurement of pancreatic islet beta-cell mass using a second-generation fluorescent exendin-4 analog.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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Authors and funding
4 authors.
Funding
Abstract
Pancreatic islet dysfunction is a key element in the development of type 2 diabetes. Determining possible early warning signs of dysfunction is thus important to determining the underlying causes of diabetes. We describe an improved fluorescent imaging approach to detect potential islet dysfunction. Using Cell Tracker Red (CTR, a mildly thiol-reactive fluorescent probe) to positively label particular islets, we measured intracellular free calcium with fura-2 AM in both CTR-labeled and unlabeled sets of pancreatic islets simultaneously in vitro. This approach enhances sensitivity by controlling for differences in background fluorescence, temperature, and perifusion dynamics. We confirmed that 200 nM CTR produced no spectral overlap with fura-2 and no significant physiological effects in selective tests of islet function. To demonstrate the utility of dual-labeling, we compared untreated islets with islets pretreated with low-dose pro-inflammatory cytokines (IL-6 + IL-1B) to induce mild dysfunction. We alternated CTR-labeling between control and test islets and identified consistent reductions in the amplitude and trajectory of glucose-stimulated calcium responses (GSCa) among cytokine-treated islets that were independent of labeling. Observations were verified using a MATLAB program specifically designed to identify key features in the GSCa. Our findings thus demonstrate the utility of CTR-labeling in identifying islet dysfunction and propose that this technique can be adapted for other cells and tissues.
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