ArticleThe Journal of biological chemistry2011
A novel putrescine importer required for type 1 pili-driven surface motility induced by extracellular putrescine in Escherichia coli K-12.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
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- Enhancement of acid tolerance of Escherichia coli by introduction of molecule chaperone CbpA from extremophile.World journal of microbiology & biotechnology · 2023Article
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- Isolation of the high polyamine-producing bacteriumBioscience of microbiota, food and health · 2023Article
- Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity.Medical sciences (Basel, Switzerland) · 2022Review
- Dickeya Manipulates Multiple Quorum Sensing Systems to Control Virulence and Collective Behaviors.Frontiers in plant science · 2022Review
- Improvement of putrescine production through the arginine decarboxylase pathway in Escherichia coli K-12.AMB Express · 2021Article
- PaeA (YtfL) protects from cadaverine and putrescine stress in Salmonella Typhimurium and E. coli.Molecular microbiology · 2021Article
- The Nutrient and Energy Pathway Requirements for Surface Motility of Nonpathogenic and UropathogenicJournal of bacteriology · 2021Article
- Effect of Spermidine on Biofilm Formation in Escherichia coli K-12.Journal of bacteriology · 2021Article
- Health-Promoting Effects of Dietary Polyamines.Medical sciences (Basel, Switzerland) · 2021Review
- DNA supercoiling differences in bacteria result from disparate DNA gyrase activation by polyamines.PLoS genetics · 2020Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, many studies have reported that polyamines play a role in bacterial cell-to-cell signaling processes. The present study describes a novel putrescine importer required for induction of type 1 pili-driven surface motility. The surface motility of the Escherichia coli ΔspeAB ΔspeC ΔpotABCD strain, which cannot produce putrescine and cannot import spermidine from the medium, was induced by extracellular putrescine. Introduction of the gene deletions for known polyamine importers (ΔpotE, ΔpotFGHI, and ΔpuuP) or a putative polyamine importer (ΔydcSTUV) into the ΔspeAB ΔspeC ΔpotABCD strain did not affect putrescine-induced surface motility. The deletion of yeeF, an annotated putative putrescine importer, in the ΔspeAB ΔspeC ΔpotABCD ΔydcSTUV strain abolished surface motility in putrescine-supplemented medium. Complementation of yeeF by a plasmid vector restored surface motility. The surface motility observed in the present study was abolished by the deletion of fimA, suggesting that the surface motility is type 1 pili-driven. A transport assay using the yeeF(+) or ΔyeeF strains revealed that YeeF is a novel putrescine importer. The K(m) of YeeF (155 μM) is 40 to 300 times higher than that of other importers reported previously. On the other hand, the V(max) of YeeF (9.3 nmol/min/mg) is comparable to that of PotABCD, PotFGHI, and PuuP. The low affinity of YeeF for putrescine may allow E. coli to sense the cell density depending on the concentration of extracellular putrescine.
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