ArticleNucleic acids research2011
A high-throughput DNA methylation analysis of a single cell.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- miR-203, a tumor suppressor frequently down-regulated by promoter hypermethylation in rhabdomyosarcoma.The Journal of biological chemistry · 2014Trial
- The crosstalk between epigenetics and metabolism in the malignant cell.Discover oncology · 2026Review
- Advances in Epigenomic Sequencing and Their Applications in Cancer Diagnostics.Precision chemistry · 2025Review
- Epigenetic Impacts of Non-Coding Mutations Deciphered Through Pre-Trained DNA Language Model at Single-Cell Resolution.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Article
- Emerging platforms for high-throughput enzymatic bioassays.Trends in biotechnology · 2023Review
- Technologies and applications of single-cell DNA methylation sequencing.Theranostics · 2023Review
- scTEM-seq: Single-cell analysis of transposable element methylation to link global epigenetic heterogeneity with transcriptional programs.Scientific reports · 2022Article
- Application of single cell genomics to focal epilepsies: A call to action.Brain pathology (Zurich, Switzerland) · 2021Review
- Precision medicine based on epigenomics: the paradigm of carcinoma of unknown primary.Nature reviews. Clinical oncology · 2017Review
- Genome-wide methylome and chromatin interactome identify abnormal enhancer to be risk factor of breast cancer.Oncotarget · 2017Article
- Bisulfite-independent analysis of CpG island methylation enables genome-scale stratification of single cells.Nucleic acids research · 2017Article
- MeDeCom: discovery and quantification of latent components of heterogeneous methylomes.Genome biology · 2017Article
- Next-generation molecular diagnosis: single-cell sequencing from bench to bedside.Cellular and molecular life sciences : CMLS · 2017Review
- Integrin α9 gene promoter is hypermethylated and downregulated in nasopharyngeal carcinoma.Oncotarget · 2015Article
- Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns.Nucleic acids research · 2015Article
- Multiplexed locus-specific analysis of DNA methylation in single cells.Nature protocols · 2015Article
- Single molecule and single cell epigenomics.Methods (San Diego, Calif.) · 2015Review
- Advances in the profiling of DNA modifications: cytosine methylation and beyond.Nature reviews. Genetics · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, the field of epigenetics has grown dramatically and has become one of the most dynamic and fast-growing branches of molecular biology. The amount of diseases suspected of being influenced by DNA methylation is rising steadily and includes common diseases such as schizophrenia, bipolar disorder, Alzheimer's disease, diabetes, atherosclerosis, cancer, major psychosis, lupus and Parkinson's disease. Due to cellular heterogeneity of methylation patterns, epigenetic analyses of single cells become a necessity. One rationale is that DNA methylation profiles are highly variable across individual cells, even in the same organ, dependent on the function of the gene, disease state, exposure to environmental factors (e.g. radiation, drugs or nutrition), stochastic fluctuations and various other causes. Using a polymerase chain reaction (PCR)-slide microreaction system, we present here a methylation-sensitive PCR analysis, the restriction enzyme-based single-cell methylation assay (RSMA), in the analysis of DNA methylation patterns in single cells. This method addresses the problems of cell heterogeneity in epigenetics research; it is comparably affordable, avoids complicated microfluidic systems and offers the opportunity for high-throughput screening, as many single cells can be screened in parallel. In addition to this study, critical principles and caveats of single cell methylation analyses are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.