Evidence map›Paper›PMID 21266468›Full record

ArticleJournal of cell science2011

Purinergic P2X7 receptor regulates lung surfactant secretion in a paracrine manner.

Amarjit Mishra, Narendranath Reddy Chintagari, Yujie Guo, Tingting Weng, Lijing Su, Lin Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Development of a peripheral blood transcriptomic gene signature to predict bronchopulmonary dysplasia.American journal of physiology. Lung cellular and molecular physiology · 2023
    Trial
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Review
  9. P2 Purinergic Signaling in the Distal Lung in Health and Disease.International journal of molecular sciences · 2020
    Review
  10. Review
  11. Review
  12. Review
  13. Observational
  14. Review
  15. ATP is stored in lamellar bodies to activate vesicular P2XThe Journal of general physiology · 2018
    Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Amarjit MishraLundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
Narendranath Reddy Chintagari
Yujie Guo
Tingting Weng
Lijing Su
Lin Liu

Funding

MOLECULAR MECHANISMS OF LUNG SURFACTANT SECRETIONR01HL052146 · NHLBI · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIU, LIN · 2000 to 2007
$2.3M
Mechanisms of Alveolar Fluid TransportR01HL083188 · NHLBI · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIU, LIN · 2006 to 2009
$1.4M
Mechanisms of Alveolar Epithelial Cell DifferentiationR01HL071628 · NHLBI · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIU, LIN · 2003 to 2006
$1.2M
NHLBI NIH HHS R01 HL-052146NHLBI NIH HHS R01 HL052146NHLBI NIH HHS R01 HL-071628NHLBI NIH HHS R01 HL071628NHLBI NIH HHS R01 HL-083188NHLBI NIH HHS R01 HL083188
6 · The paper itself

Abstract

Alveolar epithelium is composed of alveolar epithelial cells of type I (AEC I) and type II (AEC II). AEC II secrete lung surfactant by means of exocytosis. P2X(7) receptor (P2X(7)R), a P2 purinergic receptor, has been implicated in the regulation of synaptic transmission and inflammation. Here, we report that P2X(7)R, which is expressed in AEC I but not AEC II, is a novel mediator for the paracrine regulation of surfactant secretion in AEC II. In primary co-cultures of AEC I and AEC II benzoyl ATP (BzATP; an agonist of P2X(7)R) increased surfactant secretion, which was blocked by the P2X(7)R antagonist Brilliant Blue G. This effect was observed in AEC II co-cultured with human embryonic kidney HEK-293 cells stably expressing rat P2X(7)R, but not when co-cultured with AEC I in which P2X(7)R was knocked down or in co-cultures of AEC I and AEC II isolated from P2X(7)R(-/-) mice. BzATP-mediated secretion involved P2Y(2) receptor signaling because it was reduced by the addition of the ATP scavengers apyrase and adenosine deaminase and the P2Y(2) receptor antagonist suramin. However, the stimulation with BzATP might also release other substances that potentially increase surfactant secretion as a greater stimulation of secretion was observed in AEC II incubated with BzATP when co-cultured with E10 or HEK-293-P2X(7)R cells than with ATP alone. P2X(7)R(-/-) mice failed to increase surfactant secretion in response to hyperventilation, pointing to the physiological relevance of P2X(7)R in maintaining surfactant homeostasis in the lung. These results suggest that the activation of P2X(7)R increases surfactant secretion by releasing ATP from AEC I and subsequently stimulating P2Y(2) receptors in AEC II.

Indexed as

Paracrine CommunicationAdenosine TriphosphateAnimalsApoproteinsCells, CulturedEpithelial CellsHEK293 CellsHumansLungMaleMiceMice, Inbred C57BLMice, KnockoutPulmonary AlveoliPulmonary Surfactant-Associated ProteinsRatsAdenosine TriphosphateApoproteinspulmonary surfactant apoproteinPulmonary Surfactant-Associated ProteinsReceptors, Purinergic P2X7

Identifiers

PMID21266468
PMCPMC3031375

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.