ArticlePloS one2011
Identification of a key amino acid of LuxS involved in AI-2 production in Campylobacter jejuni.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- Co-Culture Reveals the Quorum-Sensing Regulatory Mechanism of Bacteriocin PlnJK Synthesis inMicroorganisms · 2026Article
- The LuxS/AI-2 quorum sensing system regulates osmotic adaptation, motility, and type VI secretion system inFrontiers in microbiology · 2026Article
- Biofilm Formation inMicroorganisms · 2023Article
- Article
- Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni.Journal of applied genetics · 2022Article
- Inhibition of AI-2 Quorum Sensing and Biofilm Formation inFrontiers in microbiology · 2021Article
- Molecular Mechanisms of Campylobacter Biofilm Formation and Quorum Sensing.Current topics in microbiology and immunology · 2021Review
- Small Noncoding RNA CjNC110 Influences Motility, Autoagglutination, AI-2 Localization, Hydrogen Peroxide Sensitivity, and Chicken Colonization in Campylobacter jejuni.Infection and immunity · 2020Article
- Adhesion, Biofilm Formation, andFrontiers in cellular and infection microbiology · 2020Article
- 2(5H)-Furanone, epigallocatechin gallate, and a citric-based disinfectant disturb quorum-sensing activity and reduce motility and biofilm formation of Campylobacter jejuni.Folia microbiologica · 2015Article
- Article
- Polyphosphate-mediated modulation of Campylobacter jejuni biofilm growth and stability.Virulence · 2014Article
- Quorum sensing circuit and reactive oxygen species resistance in Deinococcus sp.Current microbiology · 2012Article
- Quorum sensing dependent phenotypes and their molecular mechanisms in Campylobacterales.European journal of microbiology & immunology · 2012Review
- Critical role of LuxS in the virulence of Campylobacter jejuni in a guinea pig model of abortion.Infection and immunity · 2012Article
- LuxS and quorum-sensing in Campylobacter.Frontiers in cellular and infection microbiology · 2012Review
- Functional definition of LuxS, an autoinducer-2 (AI-2) synthase and its role in full virulence of Streptococcus suis serotype 2.Journal of microbiology (Seoul, Korea) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Autoinducer-2 (AI-2) mediated quorum sensing has been associated with the expression of virulence factors in a number of pathogenic organisms and has been demonstrated to play a role in motility and cytolethal distending toxin (cdt) production in Campylobacter jejuni. We have initiated the work to determine the molecular basis of AI-2 synthesis and the biological functions of quorum sensing in C. jejuni. In this work, two naturally occurring variants of C. jejuni 81116 were identified, one producing high-levels of AI-2 while the other is defective in AI-2 synthesis. Sequence analysis revealed a G92D mutation in the luxS gene of the defective variant. Complementation of the AI-2(-) variant with a plasmid encoded copy of the wild-type luxS gene or reversion of the G92D mutation by site-directed mutagenesis fully restored AI-2 production by the variant. These results indicate that the G92D mutation alone is responsible for the loss of AI-2 activity in C. jejuni. Kinetic analyses showed that the G92D LuxS has a ∼100-fold reduced catalytic activity relative to the wild-type enzyme. Findings from this study identify a previously undescribed amino acid that is essential for AI-2 production by LuxS and provide a unique isogenic pair of naturally occurring variants for us to dissect the functions of AI-2 mediated quorum sensing in Campylobacter.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.