ArticlePloS one2011
Whole-genome analysis reveals that active heat shock factor binding sites are mostly associated with non-heat shock genes in Drosophila melanogaster.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 83 citations in OpenAlex.
- Disentangling the reproductive and metabolic transcriptional responses to diet in Drosophila melanogaster.G3 (Bethesda, Md.) · 2026Article
- Inferring fungal cis-regulatory networks from genome sequences via unsupervised and interpretable representation learning.Genetics · 2026Article
- Moesin contributes to heat shock gene response through direct binding to the Med15 subunit of the Mediator complex in the nucleus.Open biology · 2024Article
- Article
- Transcriptional responses of cancer cells to heat shock-inducing stimuli involve amplification of robust HSF1 binding.Nature communications · 2023Article
- AiScience · 2023Article
- Wds-Mediated H3K4me3 Modification Regulates Lipid Synthesis and Transport inInternational journal of molecular sciences · 2023Article
- Heat-Induced Conformational Transition Mechanism of Heat Shock Factor 1 Investigated by Tryptophan Probe.Biochemistry · 2022Article
- Transcription dynamics of heat shock proteins in response to thermal acclimation inFrontiers in physiology · 2022Article
- A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells.BMC genomics · 2021Article
- Evolution and genomic organization of the insect sHSP gene cluster and coordinate regulation in phenotypic plasticity.BMC ecology and evolution · 2021Article
- Heat Shock Causes LowerFrontiers in immunology · 2021Article
- Cloning and identification of grass carp transcription factor HSF1 and its characterization involving the production of fish HSP70.Fish physiology and biochemistry · 2020Article
- Heat Shock Factor Is Involved in Regulating the Transcriptional Expression of Two Potential Hsps (Frontiers in physiology · 2020Article
- Structural analysis of missense mutations occurring in the DNA-binding domain of HSF4 associated with congenital cataracts.Journal of structural biology: X · 2020Article
- Diversification of theeLife · 2019Article
- Comparison between transcriptomic responses to short-term stress exposures of a common Holarctic and endemic Lake Baikal amphipods.BMC genomics · 2019Article
- A role for heat shock factor 1 in hypercapnia-induced inhibition of inflammatory cytokine expression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Evolutionary Origin, Gradual Accumulation and Functional Divergence ofFrontiers in plant science · 2018Article
- Transcriptional Regulation of the Ambient Temperature Response by H2A.Z Nucleosomes and HSF1 Transcription Factors in Arabidopsis.Molecular plant · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During heat shock (HS) and other stresses, HS gene transcription in eukaryotes is up-regulated by the transcription factor heat shock factor (HSF). While the identities of the major HS genes have been known for more than 30 years, it has been suspected that HSF binds to numerous other genes and potentially regulates their transcription. In this study, we have used a chromatin immunoprecipitation and microarray (ChIP-chip) approach to identify 434 regions in the Drosophila genome that are bound by HSF. We have also performed a transcript analysis of heat shocked Kc167 cells and third instar larvae and compared them to HSF binding sites. The heat-induced transcription profiles were quite different between cells and larvae and surprisingly only about 10% of the genes associated with HSF binding sites show changed transcription. There were also genes that showed changes in transcript levels that did not appear to correlate with HSF binding sites. Analysis of the locations of the HSF binding sites revealed that 57% were contained within genes with approximately 2/3rds of these sites being in introns. We also found that the insulator protein, BEAF, has enriched binding prior to HS to promoters of genes that are bound by HSF upon HS but that are not transcriptionally induced during HS. When the genes associated with HSF binding sites in promoters were analyzed for gene ontology terms, categories such as stress response and transferase activity were enriched whereas analysis of genes having HSF binding sites in introns identified those categories plus ones related to developmental processes and reproduction. These results suggest that Drosophila HSF may be regulating many genes besides the known HS genes and that some of these genes may be regulated during non-stress conditions.
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