ArticlePlant physiology2011
Resolving the compartmentation and function of C4 photosynthesis in the single-cell C4 species Bienertia sinuspersici.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- A novel single-cell NAD-ME CThe New phytologist · 2026Article
- Molecular phylogenetics and evolutionary history ofFrontiers in plant science · 2026Review
- NAD(H) and NADP(H) in plants and mammals.Molecular plant · 2025Review
- Investigating theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Investigating thebioRxiv : the preprint server for biology · 2024Article
- Diversification of Plastid Structure and Function in Land Plants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Expression and localization of two β-carbonic anhydrases inFrontiers in plant science · 2024Article
- Chloroplast Envelopes Play a Role in the Formation of Autophagy-Related Structures in Plants.Plants (Basel, Switzerland) · 2023Article
- Article
- Differential regulation of reactive oxygen species in dimorphic chloroplasts of single cell CFrontiers in plant science · 2023Article
- Development of C4 Biochemistry and Change in Expression of Markers for Photosystems I and II in the Single-Cell C4 Species, Bienertia sinuspersiciPlants (Basel, Switzerland) · 2022Article
- Electron Microscopy Views of Dimorphic Chloroplasts in C4 Plants.Frontiers in plant science · 2020Review
- The Developmental Enhancement of a CFrontiers in plant science · 2020Article
- Methods of analysis of chloroplast genomes of CPlant methods · 2020Article
- A draft genome assembly of halophyte Suaeda aralocaspica, a plant that performs C4 photosynthesis within individual cells.GigaScience · 2019Article
- Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts.Plant cell reports · 2019Article
- EfficientFrontiers in plant science · 2019Article
- The intracellular distribution of inorganic carbon fixing enzymes does not support the presence of a C4 pathway in the diatom Phaeodactylum tricornutum.Photosynthesis research · 2018Article
- Transit peptide elements mediate selective protein targeting to two different types of chloroplasts in the single-cell C4 species Bienertia sinuspersici.Scientific reports · 2017Article
- A MEM1-like motif directs mesophyll cell-specific expression of the gene encoding the C4 carbonic anhydrase in Flaveria.Journal of experimental botany · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bienertia sinuspersici is a land plant known to perform C(4) photosynthesis through the location of dimorphic chloroplasts in separate cytoplasmic domains within a single photosynthetic cell. A protocol was developed with isolated protoplasts to obtain peripheral chloroplasts (P-CP), a central compartment (CC), and chloroplasts from the CC (C-CP) to study the subcellular localization of photosynthetic functions. Analyses of these preparations established intracellular compartmentation of processes to support a NAD-malic enzyme (ME)-type C(4) cycle. Western-blot analyses indicated that the CC has Rubisco from the C(3) cycle, the C(4) decarboxylase NAD-ME, a mitochondrial isoform of aspartate aminotransferase, and photorespiratory markers, while the C-CP and P-CP have high levels of Rubisco and pyruvate, Pidikinase, respectively. Other enzymes for supporting a NAD-ME cycle via an aspartate-alanine shuttle, carbonic anhydrase, phosophoenolpyruvate carboxylase, alanine, and an isoform of aspartate aminotransferase are localized in the cytosol. Functional characterization by photosynthetic oxygen evolution revealed that only the C-CP have a fully operational C(3) cycle, while both chloroplast types have the capacity to photoreduce 3-phosphoglycerate. The P-CP were enriched in a putative pyruvate transporter and showed light-dependent conversion of pyruvate to phosphoenolpyruvate. There is a larger investment in chloroplasts in the central domain than in the peripheral domain (6-fold more chloroplasts and 4-fold more chlorophyll). The implications of this uneven distribution for the energetics of the C(4) and C(3) cycles are discussed. The results indicate that peripheral and central compartment chloroplasts in the single-cell C(4) species B. sinuspersici function analogous to mesophyll and bundle sheath chloroplasts of Kranz-type C(4) species.
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