ArticleThe Journal of biological chemistry2011
Halotolerant cyanobacterium Aphanothece halophytica contains an Na+-dependent F1F0-ATP synthase with a potential role in salt-stress tolerance.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 44 citations in OpenAlex.
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- Global transcriptome analyses and regulatory mechanisms in Halothece sp. PCC 7418 exposed to abiotic stresses.Applied microbiology and biotechnology · 2022Article
- Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418.Scientific reports · 2022Article
- Transcriptome Analysis Reveals Molecular Mechanisms under Salt Stress in Leaves of Foxtail Millet (Plants (Basel, Switzerland) · 2022Article
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- Enrichment and physiological characterization of a novel comammox Nitrospira indicates ammonium inhibition of complete nitrification.The ISME journal · 2021Article
- The Effects of Salts and Osmoprotectants on Enzyme Activities of Fructose-1,6-biphosphate Aldolases in a Halotolerant Cyanobacterium,Life (Basel, Switzerland) · 2020Article
- Improved Salt Tolerance and Metabolomics Analysis ofFrontiers in bioengineering and biotechnology · 2020Article
- Functional Gene Expression in Shark Bay Hypersaline Microbial Mats: Adaptive Responses.Frontiers in microbiology · 2020Article
- Short-Term Temporal Metabolic Behavior in Halophilic CyanobacteriumMetabolites · 2019Article
- Halophiles and Their Vast Potential in Biofuel Production.Frontiers in microbiology · 2019Review
- COGcollator: a web server for analysis of distant relationships between homologous protein families.Biology direct · 2017Article
- Molecular architecture of the N-type ATPase rotor ring fromEMBO reports · 2017Article
- Speciation and ecological success in dimly lit waters: horizontal gene transfer in a green sulfur bacteria bloom unveiled by metagenomic assembly.The ISME journal · 2017Article
- Glucose Uptake inFrontiers in microbiology · 2017Article
- Eukaryotic G protein-coupled receptors as descendants of prokaryotic sodium-translocating rhodopsins.Biology direct · 2015Article
- Ancient Systems of Sodium/Potassium Homeostasis as Predecessors of Membrane Bioenergetics.Biochemistry. Biokhimiia · 2015Article
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7 authors at 3 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H(+)-ATP synthase, Aphanothece halophytica contains a putative F(1)F(0)-type Na(+)-ATP synthase (ApNa(+)-ATPase) operon (ApNa(+)-atp). The operon consists of nine genes organized in the order of putative subunits β, ε, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa(+)-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa(+)-ATPase exhibited Na(+)-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na(+) ion protected the inhibition of ApNa(+)-ATPase by N,N'-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na(+)-loaded inverted membrane vesicles. Expression of the ApNa(+)-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na(+)-dependent F(1)F(0)-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.
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