ArticleProceedings of the National Academy of Sciences of the United States of America2011
Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins.Membranes · 2016Article
- Polylysine-mediated translocation of the diphtheria toxin catalytic domain through the anthrax protective antigen pore.Biochemistry · 2014Article
- Binding of MgtR, a Salmonella transmembrane regulatory peptide, to MgtC, a Mycobacterium tuberculosis virulence factor: a structural study.Journal of molecular biology · 2014Article
- Small molecule inhibitors of anthrax lethal factor toxin.Bioorganic & medicinal chemistry · 2014Article
- Anthrax toxin-induced rupture of artificial lipid bilayer membranes.The Journal of chemical physics · 2013Article
- Three dimensional structure of the anthrax toxin translocon-lethal factor complex by cryo-electron microscopy.Protein science : a publication of the Protein Society · 2013Article
- Assembly of anthrax toxin pore: lethal-factor complexes into lipid nanodiscs.Protein science : a publication of the Protein Society · 2013Article
- Ratcheting up protein translocation with anthrax toxin.Protein science : a publication of the Protein Society · 2012Review
- Ultrasensitive detection of protein translocated through toxin pores in droplet-interface bilayers.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Cys-Cys cross-linking shows contact between the N-terminus of lethal factor and Phe427 of the anthrax toxin pore.Biochemistry · 2011Article
- The unfolding story of anthrax toxin translocation.Molecular microbiology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The protective antigen (PA) moiety of anthrax toxin forms oligomeric pores that translocate the enzymatic moieties of the toxin--lethal factor (LF) and edema factor (EF)--across the endosomal membrane of mammalian cells. Here we describe site-directed spin-labeling studies that identify interactions of LF with the prepore and pore conformations of PA. Our results reveal a direct interaction between the extreme N terminus of LF (residues 2-5) and the Φ-clamp, a structure within the lumen of the pore that catalyzes translocation. Also, consistent with a recent crystallographic model, we find that, upon binding of the translocation substrate to PA, LF helix α1 separates from helices α2 and α3 and binds in the α-clamp of PA. These interactions, together with the binding of the globular part of the N-terminal domain of LF to domain 1' of PA, indicate that LF interacts with the PA pore at three distinct sites. Our findings elucidate the state from which translocation of LF and EF proceeds through the PA pore.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.