ArticleProceedings of the National Academy of Sciences of the United States of America2011
Different nuclease requirements for exosome-mediated degradation of normal and nonstop mRNAs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- A meta-analysis of single base-pair substitutions in translational termination codons ('nonstop' mutations) that cause human inherited disease.Human genomics · 2011Pooled it
- A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay.Genes · 2024Article
- The physiological and pathological mechanisms of early embryonic development.Fundamental research · 2022Review
- The human SKI complex regulates channeling of ribosome-bound RNA to the exosome via an intrinsic gatekeeping mechanism.Molecular cell · 2022Article
- Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Ewing sarcoma family of tumors-derived small extracellular vesicle proteomics identify potential clinical biomarkers.Oncotarget · 2020Article
- Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis.BMC genomics · 2020Article
- NONU-1 Encodes a Conserved Endonuclease Required for mRNA Translation Surveillance.Cell reports · 2020Article
- Ribosome-based quality control of mRNA and nascent peptides.Wiley interdisciplinary reviews. RNA · 2017Review
- The RNA Exosome Channeling and Direct Access Conformations Have Distinct In Vivo Functions.Cell reports · 2016Article
- Chromatin Dynamics and the RNA Exosome Function in Concert to Regulate Transcriptional Homeostasis.Cell reports · 2015Article
- Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.Biology of reproduction · 2015Article
- The ribonuclease DIS3 promotes let-7 miRNA maturation by degrading the pluripotency factor LIN28B mRNA.Nucleic acids research · 2015Article
- RNA exosome-regulated long non-coding RNA transcription controls super-enhancer activity.Cell · 2015Article
- The RNA exosome affects iron response and sensitivity to oxidative stress.RNA (New York, N.Y.) · 2014Article
- Protecting the proteome: Eukaryotic cotranslational quality control pathways.The Journal of cell biology · 2014Review
- Proteins involved in the degradation of cytoplasmic mRNA in the major eukaryotic model systems.RNA biology · 2014Review
- The intimate relationships of mRNA decay and translation.Trends in genetics : TIG · 2013Review
- Threading the barrel of the RNA exosome.Trends in biochemical sciences · 2013Review
- Emerging roles for ribonucleoprotein modification and remodeling in controlling RNA fate.Trends in cell biology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Two general pathways of mRNA decay have been characterized in yeast. In one pathway, the mRNA is degraded by the cytoplasmic form of the exosome. The exosome has both 3' to 5' exoribonuclease and endoribonuclease activity, and the available evidence suggests that the exonuclease activity is required for the degradation of mRNAs. We confirm here that this is true for normal mRNAs, but that aberrant mRNAs that lack a stop codon can be efficiently degraded in the absence of the exonuclease activity of the exosome. Specifically, we show that the endo- and exonuclease activities of the exosome are both capable of rapidly degrading nonstop mRNAs and ribozyme-cleaved mRNAs. Additionally, the endonuclease activity of the exosome is not required for endonucleolytic cleavage in no-go decay. In vitro, the endonuclease domain of the exosome is active only under nonphysiological conditions, but our findings show that the in vivo activity is sufficient for the rapid degradation of nonstop mRNAs. Thus, whereas normal mRNAs are degraded by two exonucleases (Xrn1p and Rrp44p), several endonucleases contribute to the decay of many aberrant mRNAs, including transcripts subject to nonstop and no-go decay. Our findings suggest that the nuclease requirements for general and nonstop mRNA decay are different, and describe a molecular function of the core exosome that is not disrupted by inactivating its exonuclease activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.