ArticleProtein expression and purification2011
New ligation-independent cloning vectors compatible with a high-throughput platform for parallel construct expression evaluation using baculovirus-infected insect cells.
Article in Protein expression and purification, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- An Alternative DNA Endonuclease Activity is Associated with the LINE-1 ORF2-encoded Protein.bioRxiv : the preprint server for biology · 2026Article
- Secretory glycoprotein NS1 plays a crucial role in the particle formation of flaviviruses.PLoS pathogens · 2022Article
- HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system.Scientific reports · 2022Article
- Structural basis for antibody inhibition of flavivirus NS1-triggered endothelial dysfunction.Science (New York, N.Y.) · 2021Article
- Safety and immunogenicity of mammalian cell derived and Modified Vaccinia Ankara vectored African swine fever subunit antigens in swine.Veterinary immunology and immunopathology · 2017Article
- A Mutation Identified in Neonatal Microcephaly Destabilizes Zika Virus NS1 Assembly in Vitro.Scientific reports · 2017Article
- Contribution of intertwined loop to membrane association revealed by Zika virus full-length NS1 structure.The EMBO journal · 2016Article
- Extended surface for membrane association in Zika virus NS1 structure.Nature structural & molecular biology · 2016Article
- Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen.Biotechnology and bioengineering · 2016Article
- Conformational Sampling and Binding Site Assessment of Suppression of Tumorigenicity 2 Ectodomain.PloS one · 2016Article
- One-pot refolding of core histones from bacterial inclusion bodies allows rapid reconstitution of histone octamer.Protein expression and purification · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Biomedical research has undergone a major shift in emphasis over the past decade from characterizing the genomes of organisms to characterizing their proteomes. The high-throughput approaches that were successfully applied to sequencing of genomes, such as miniaturization and automation, have been adapted for high-throughput cloning and protein production. High-throughput platforms allow for a multi-construct, multi-parallel approach to expression optimization and construct evaluation. We describe here a series of baculovirus transfer and expression vectors that contain ligation-independent cloning regions originally designed for use in high-throughput Escherichia coli expression evaluation. These new vectors allow for parallel cloning of the same gene construct into a variety of baculovirus or E. coli expression vectors. A high-throughput platform for construct expression evaluation in baculovirus-infected insect cells was developed to utilize these vectors. Data from baculovirus infection expression trials for multiple constructs of two target protein systems relevant to the study of human diseases are presented. The target proteins exhibit a wide variation in behavior and illustrate the benefit of investigating multiple cell types, fusion partners and secretion signals in optimization of constructs and conditions for eukaryotic protein production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.